Chang Yen, Lai Po-Hong, Wei Hao-Ji, Lin Wei-Wen, Chen Chun-Hung, Hwang Shiaw-Min, Chen Sung-Ching, Sung Hsing-Wen
Division of Cardiovascular Surgery, Veterans General Hospital-Taichung and College of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC.
J Thorac Cardiovasc Surg. 2007 Jul;134(1):65-73, 73.e1-4. doi: 10.1016/j.jtcvs.2007.02.019.
We sought to induce tissue regeneration within a porous patch for repair of a myocardial defect.
A basic fibroblast growth factor-loaded porous bovine pericardium populated with 5-bromo-2'-deoxyuridine-labeled mesenchymal stem cells was used as a cardiac patch (the basic fibroblast growth factor/mesenchymal stem cell patch) to repair a defect created in a syngeneic rat model. The blank porous pericardium (the control patch) and the patch loaded with basic fibroblast growth factor were used as controls. The implanted patches were retrieved at 4 and 12 weeks postoperatively (n = 5 per group at each time point).
At retrieval, we found that none of the patches were thinned or dilated. Endothelialization and remesothelialization were observed on the endocardial and epicardial surfaces of patches in each of the studied groups, respectively. Additionally, newly regenerated muscle fibers, glycosaminoglycans, smooth muscle cells, and microvessels were seen in the middle layers of all patches, an indication of tissue regeneration. However, the extents of tissue regeneration in the basic fibroblast growth factor and basic fibroblast growth factor/mesenchymal stem cell patches were more pronounced than in those of the control patch. This may be attributed to the fact that the densities of neomicrovessels observed in the basic fibroblast growth factor and basic fibroblast growth factor/mesenchymal stem cell patches were significantly greater than in those of the control patch. 5-Bromo-2'-deoxyuridine-labeled cardiomyocytes, smooth muscle cells, and endothelial cells were identified in the basic fibroblast growth factor/mesenchymal stem cells patch, and no cardiomyocytes were observed in the control and basic fibroblast growth factor patches.
The results provided evidence of tissue regeneration within a porous bovine pericardium through a process involving cell recruitment and tissue-specific differentiation.
我们试图在多孔补片中诱导组织再生以修复心肌缺损。
将负载碱性成纤维细胞生长因子且接种了5-溴-2'-脱氧尿苷标记的间充质干细胞的多孔牛心包用作心脏补片（碱性成纤维细胞生长因子/间充质干细胞补片），以修复同基因大鼠模型中制造的缺损。空白多孔心包（对照补片）和负载碱性成纤维细胞生长因子的补片用作对照。术后4周和12周取出植入的补片（每个时间点每组n = 5）。
取出时，我们发现所有补片均未变薄或扩张。在各研究组补片的心内膜和心外膜表面分别观察到内皮化和间皮修复。此外，在所有补片的中层均可见新再生的肌纤维、糖胺聚糖、平滑肌细胞和微血管，这表明有组织再生。然而，碱性成纤维细胞生长因子补片和碱性成纤维细胞生长因子/间充质干细胞补片中的组织再生程度比对照补片中更明显。这可能归因于在碱性成纤维细胞生长因子补片和碱性成纤维细胞生长因子/间充质干细胞补片中观察到的新生微血管密度明显高于对照补片。在碱性成纤维细胞生长因子/间充质干细胞补片中鉴定出5-溴-2'-脱氧尿苷标记的心肌细胞、平滑肌细胞和内皮细胞，而在对照补片和碱性成纤维细胞生长因子补片中未观察到心肌细胞。
结果提供了证据，表明多孔牛心包内通过细胞募集和组织特异性分化过程实现了组织再生。
