中耳炎患儿中耳积液中胃蛋白酶的检测

Detection of gastric pepsin in middle ear fluid of children with otitis media.

作者信息

He Zhaoping, O'Reilly Robert C, Bolling Laura, Soundar Sam, Shah Mansi, Cook Steven, Schmidt Richard J, Bloedon Esa, Mehta Devendra I

机构信息

Nemours Biomedical Research, Alfred I. duPont Hospital for Children, Wilmington, DE 19899, USA.

出版信息

Otolaryngol Head Neck Surg. 2007 Jul;137(1):59-64. doi: 10.1016/j.otohns.2007.02.002.

DOI:10.1016/j.otohns.2007.02.002

PMID:17599566

Abstract

OBJECTIVE

We sought to confirm the finding of pepsin/pepsinogen in the middle ear fluid of children with otitis media in a larger sample size using a sensitive and specific pepsin assay.

STUDY DESIGN AND SETTING

We evaluated 152 children (225 ear samples) in a prospective study at a tertiary care children's hospital. The presence of pepsin in middle ear aspirates was determined using enzymatic assay.

RESULTS

Of the patients, 14.4 percent (22 of 152) had detectable pepsin activity in one or both of the ear samples with no pepsin activity detected in control serum. Average pepsin concentration in the samples was 96.6 +/- 170.8 ng/ml, ranging from 13 to 687 ng/ml. Pepsin concentration in the middle ear of children younger than 1.0 year was significantly higher than in older age groups.

CONCLUSION AND SIGNIFICANCE

Results indicate that pepsin/pepsinogen is present in the middle ears of children with otitis media, although not at the high rate previously reported. Gastric reflux may be one causative factor in the pathogenesis of otitis media.

摘要