Yoo Hye Hyun, Park Jeong Hill, Kwon Sung Won
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University; Seoul 151-742, Korea.
Phytother Res. 2007 Sep;21(9):900-3. doi: 10.1002/ptr.2213.
The goal of this study was to examine the in vitro antiproliferative activity of crude methanol extracts of three traditional Korean medicinal plants: Achyranthes fauriei, Epimedium koreanum Nakai and Scutellaria baicalensis before and after heat processing. The extracts were screened for antitumoral potential by means of an MTT assay on four human cancer cell lines: lung cancer cells (Lu1), colon cancer cells (Col2), oral epidermoid carcinomas (KB) and hormone-dependent prostate cancer cells (LNCaP). None of the extracts showed significant activity against any of the cancer cell lines. However, after treatment with steam, the processed Achyranthes fauriei extract exhibited a slight, but enhanced cytotoxic activity against all four of the cancer cell lines. The processed Scutellaria baicalensis extract exerted a potent cytotoxic activity against the Lu1 cell line in a specific manner with an IC(50) value of 14.3 microg/mL. The Epimedium koreanum extract showed no cytotoxic effects against any of the cancer cell lines with or without heat processing. These results suggest that the heat processing of medicinal plants represents a possible route to the development of antitumor agents.
本研究的目的是检测三种传统韩国药用植物牛膝、淫羊藿和黄芩生品及炮制品的甲醇粗提物的体外抗增殖活性。通过MTT法在四种人类癌细胞系上筛选提取物的抗肿瘤潜力，这四种细胞系分别为肺癌细胞（Lu1）、结肠癌细胞（Col2）、口腔表皮样癌细胞（KB）和激素依赖性前列腺癌细胞（LNCaP）。所有提取物对任何癌细胞系均未显示出显著活性。然而，经蒸汽处理后，炮制后的牛膝提取物对所有四种癌细胞系均表现出轻微但增强的细胞毒性活性。炮制后的黄芩提取物对Lu1细胞系具有显著的细胞毒性活性，IC(50)值为14.3μg/mL。淫羊藿提取物无论是否经过热处理，对任何癌细胞系均未显示出细胞毒性作用。这些结果表明，药用植物的热处理可能是开发抗肿瘤药物的一条途径。
