Departamento de Alimentos e Toxicologia, Faculdade de Farmácia e Bioquímica, Universidade Federal de Juiz de Fora, Martelos, CEP 36036-330, Juiz de Fora, MG, Brazil.
Laboratório de Substâncias Antitumorais, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, CEP 31270-901, Belo Horizonte, MG, Brazil.
Int J Mol Sci. 2009 Aug 26;10(9):3713-3721. doi: 10.3390/ijms10093713.
In the present study, the phenolic (Folin-Dennis) and flavonoid (colorimetric assay) constituents, antioxidant [2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) assay] and cytotoxic activities of an aqueous extract (AE) of Centella asiatica leaves were investigated. The aqueous extract (50 g/L) was obtained by infusion followed by cold maceration for 24 h. The levels of phenolic and flavonoid compounds were 2.86 g/100 g and 0.361 g/100 g, respectively. The AE showed elevated DPPH scavenging activity, with an IC(50) value of 31.25 microg/mL. The AE had a promising activity against mouse melanoma (B(16)F(1)), human breast cancer (MDA MB-231) and rat glioma (C(6)) cell lines, with IC(50) values of 698.0, 648.0 and 1000.0 microg/mL, respectively. A positive correlation was established between the level of flavonoids, antioxidant and antitumor activities.
在本研究中,对积雪草叶的水提物(AE)的酚类(福林-丹尼斯)和类黄酮(比色法)成分、抗氧化(2,2-二苯基-1-苦肼基自由基(DPPH)测定法)和细胞毒性活性进行了研究。通过浸渍并随后在冷浸 24 小时获得水提取物(50 g/L)。酚类和类黄酮化合物的水平分别为 2.86 g/100 g 和 0.361 g/100 g。AE 显示出升高的 DPPH 清除活性,IC(50)值为 31.25 微克/毫升。AE 对小鼠黑色素瘤(B(16)F(1))、人乳腺癌(MDA MB-231)和大鼠神经胶质瘤(C(6))细胞系具有有前途的活性,IC(50)值分别为 698.0、648.0 和 1000.0 微克/毫升。黄酮类化合物、抗氧化和抗肿瘤活性之间建立了正相关关系。