Govindarajan R, Vijayakumar M, Rao Ch V, Shirwaikar A, Kumar Santhosh, Rawat A K S, Pushpangadan P
Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow 226001, India.
Phytother Res. 2007 Oct;21(10):975-9. doi: 10.1002/ptr.2199.
Flavonoid and alkaloid fractions of Desmodium gangeticum were evaluated for antiinflammatory and antioxidant activities in carrageenan-induced inflamed rats with the aim of studying the promising fraction for inhibitory action on ferrous sulphate induced lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and total reduced glutathione in liver and spleen homogenates of inflamed rats. The results showed that the flavonoid fraction of D. gangeticum possesses potent antioxidant activity compared with the alkaloid fraction and also with respect to the standard drug indomethacin, in terms of augmentation of the liver and spleen SOD, CAT and GPX activities, concomitant with a reduction in lipid peroxidation (TBARS). In addition, the quantification of caffeic and chlorogenic acid in the flavonoid fraction has also been carried out using HPLC, which can be utilized as a marker compound in the standardization.
对恒河荩草的黄酮类和生物碱部分进行了评估，以研究其在角叉菜胶诱导的炎症大鼠中的抗炎和抗氧化活性，目的是找出对硫酸亚铁诱导的脂质过氧化、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPX）以及炎症大鼠肝脏和脾脏匀浆中总还原型谷胱甘肽具有抑制作用的有效部分。结果表明，与生物碱部分以及标准药物吲哚美辛相比，恒河荩草的黄酮类部分具有强大的抗氧化活性，表现为肝脏和脾脏中SOD、CAT和GPX活性增强，同时脂质过氧化（TBARS）减少。此外，还使用高效液相色谱法对黄酮类部分中的咖啡酸和绿原酸进行了定量，其可作为标准化中的标记化合物。
