Yang Yi, Zhang Fan
College of Science, Beijing University of Chemical Technology, Beijing 100029, China.
College of Science, Beijing University of Chemical Technology, Beijing 100029, China.
Ultrason Sonochem. 2008 Apr;15(4):308-313. doi: 10.1016/j.ultsonch.2007.05.001. Epub 2007 May 13.
An ultrasonic method for the extractions of rutin and quercetin from Euonymus alatus (Thunb.) Sieb was investigated. The influence of four extraction variables on extraction yield of rutin and quercetin was discussed. The optimum extraction conditions found were: 70% aqueous ethanol; solvent: sample ratio 40:1 (v/w); extraction time 3 x 30 min. The recovery of rutin and quercetin and the reproducibility of the extraction method were determined. The optimized ultrasonic extraction conditions were applied to extract rutin and quercetin from dried stalks of E. alatus (Thunb.) Sieb. The application of sonication method was shown to be highly efficient in the extraction of rutin and quercetin from E. alatus (Thunb.) Sieb, compared with classical methods. The scanning electron microscopy (SEM) micrographs provided evidence of more rapid opening of plant cells treated by UAE in contrast to maceration.
研究了一种从翅卫矛中提取芦丁和槲皮素的超声方法。讨论了四个提取变量对芦丁和槲皮素提取率的影响。发现的最佳提取条件为：70%乙醇水溶液；溶剂与样品比例40:1（v/w）；提取时间3×30分钟。测定了芦丁和槲皮素的回收率以及提取方法的重现性。将优化的超声提取条件应用于从翅卫矛干燥茎中提取芦丁和槲皮素。与传统方法相比，超声处理法在从翅卫矛中提取芦丁和槲皮素方面显示出高效性。扫描电子显微镜（SEM）显微照片提供了证据，表明与浸渍法相比，经超声辅助提取（UAE）处理的植物细胞能更快地打开。
