[高效液相色谱法测定卫矛中香豆素]
[Determination of aromadendrin in Euonymus alatus by HPLC].
作者信息
Chen Yunhua, Gong Muxin, Zhang Chao, Song Yafang, Yu Ping
机构信息
College of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2010 Oct;35(19):2565-7.
OBJECTIVE
To establish a HPLC method to determine the content of aromadendrin in Euonymus alatus.
METHOD
Kromasil 100-5C18 column was used with a mobile phase composed of acetonitrile-0.3% glacial acetic acid (24:76) at a flow rate of 1.0 mL x min(-1). The detection wavelength was 292 nm and the temperature of column was 30 degrees C.
RESULT
The calibration curve was linear in the range of 0.051-2.04 microg for aromadendrin. The correlation coefficient of the calibration curve was 0.999 9. Twenty batches of the crude E. alatus purchased from different areas were determined and the contents of aromadendrin in the twigs of E. alatus were fluctuated from 0.001 57% to 0.041 6%.
CONCLUSION
This method is simple, repeatable and can be used for determination of aromadendrin.
目的
建立一种测定翅卫矛中香叶木素含量的高效液相色谱法。
方法
采用Kromasil 100 - 5C18色谱柱，以乙腈 - 0.3%冰醋酸（24:76）为流动相，流速为1.0 mL·min⁻¹。检测波长为292 nm，柱温为30℃。
结果
香叶木素在0.051 - 2.04 μg范围内校准曲线呈线性。校准曲线的相关系数为0.999 9。对从不同地区购买的20批翅卫矛药材进行测定，翅卫矛嫩枝中香叶木素的含量在0.001 57%至0.041 6%之间波动。
结论
该方法简便、可重复，可用于香叶木素的含量测定。
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