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铒铬:钇钪镓石榴石激光结合径向发射头对感染粪肠球菌的根管牙本质壁的抗菌效果。

The antimicrobial efficacy of the erbium, chromium:yttrium-scandium-gallium-garnet laser with radial emitting tips on root canal dentin walls infected with Enterococcus faecalis.

作者信息

Gordon Wanda, Atabakhsh Vahid A, Meza Fernando, Doms Aaron, Nissan Roni, Rizoiu Ioana, Stevens Roy H

机构信息

Department of Endodontology, Temple University, Philadelphia, USA.

出版信息

J Am Dent Assoc. 2007 Jul;138(7):992-1002. doi: 10.14219/jada.archive.2007.0297.

Abstract

BACKGROUND

The authors used an in vitro model to investigate the ability of an erbium, chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser with radial emitting tips to disinfect Enterococcus faecalis-infected dentin.

MATERIALS AND METHODS

The in vitro infected-dentin model system consisted of a dentin cylinder, prepared from a human anterior tooth root, cemented into a sealable two-chamber device fabricated from a syringe needle cap. The model's lower chamber contained a buffer solution, and the dentin cylinder was placed between the upper and lower chambers. After sterilization, the authors inoculated the root canal of each dentin cylinder with E. faecalis. They used an Er,Cr:YSGG laser with radial emitting tips to irradiate the root canal of each infected dentin cylinder (varying laser power and exposure time). After laser treatment, the authors machined the root canal dentin walls and collected the resulting dentin fillings in the buffer-reservoir. They quantified the E. faecalis titer of each buffer-reservoir by using selective agar plates.

RESULTS

The authors found that bacterial recovery decreased when laser irradiation duration or power increased. A greater degree of disinfection was achieved with a 120-second application of laser than with sodium hypochlorite treatment. Finally, they found that a 99.7 percent reduction in bacterial counts could be obtained using the laser.

CONCLUSION

The results of this study suggest that the Er,Cr:YSGG laser with a radial emitting tip has a significant antimicrobial effect on dentinal tubules infected with E. faecalis.

CLINICAL IMPLICATIONS

Er,Cr:YSGG laser treatment could be a valuable tool for root canal disinfection during endodontic treatment.

摘要

背景

作者使用体外模型研究了配备径向发射头的铒铬:钇钪镓石榴石(Er,Cr:YSGG)激光对粪肠球菌感染牙本质进行消毒的能力。

材料与方法

体外感染牙本质模型系统由一个牙本质圆柱体组成,该圆柱体由人类前牙根制备而成,粘结在一个由注射器针头帽制成的可密封双腔装置中。模型的下腔包含缓冲溶液,牙本质圆柱体置于上下腔之间。灭菌后,作者将粪肠球菌接种到每个牙本质圆柱体的根管中。他们使用配备径向发射头的Er,Cr:YSGG激光照射每个感染牙本质圆柱体的根管(改变激光功率和照射时间)。激光治疗后,作者对根管牙本质壁进行加工,并将得到的牙本质填充物收集到缓冲液储存器中。他们通过使用选择性琼脂平板对每个缓冲液储存器中的粪肠球菌滴度进行定量。

结果

作者发现,随着激光照射时间或功率的增加,细菌回收率降低。与次氯酸钠处理相比,激光照射120秒能实现更大程度的消毒。最后,他们发现使用激光可使细菌数量减少99.7%。

结论

本研究结果表明,配备径向发射头的Er,Cr:YSGG激光对感染粪肠球菌的牙本质小管具有显著的抗菌作用。

临床意义

Er,Cr:YSGG激光治疗可能是牙髓治疗中根管消毒的一种有价值的工具。

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