Dipartimento delle Discipline Chirurgiche, Oncologiche e Stomatologiche (Di.Chir.On.S), University of Palermo, Via del Vespro 129, 90127, Palermo, Italy,
Lasers Med Sci. 2015 Feb;30(2):707-12. doi: 10.1007/s10103-013-1410-6. Epub 2013 Aug 6.
Some lasers have demonstrated to provide effective disinfection when used as adjunctive device to the conventional treatment. The aim of this in vitro study was to determine the effectiveness of the erbium, chromium:yttrium scandium gallium garnet (Er, Cr:YSGG) laser by measuring its bactericidal effect inside the root canal experimentally colonized with Enterococcus faecalis. The laser was tested at different irradiation times (30 and 60 s) and energy of impulses (75 and 25 mJ). A total of 52 single-rooted extracted human teeth were endodontically prepared with rotary instrumentation. All were sterilized and inoculated with a suspension of E. faecalis (105 bacteria/ml). The teeth were randomized into three treatment (group 1, group 2, and group 3) and one control groups. In all groups, teeth were chemically irrigated with 5.25% sodium hypochlorite and 17% ethylenediaminetetraacetic acid. Groups 1 and 2 were also irradiated at 30 and 60 s, respectively, with an Er, Cr:YSGG laser at 75 mJ. Teeth of group 3 were treated with laser for 60 s at 25 mJ. Samples were processed to detect the presence of E. faecalis. For all groups, a bactericidal effect was observed. The use of laser at 75 mJ with an irradiation time of 30 and 60 s eliminated a percentage of 92.3 and 100% of E. faecalis, respectively. In the control group, a reduction of 92.3% was observed. Lower percentage of reduction (46.1%) was obtained in teeth treated with laser at 25 mJ for 60 s. No statistical differences were observed between the groups (P = 0.543, Fisher's exact test). The results indicated a bactericidal effect of Er, Cr:YSGG laser irradiation at the settings used in this study. The highest bactericidal effect of this laser was observed at 60 s of irradiation time, using an energy pulse of 75 mJ.
一些激光已被证明在作为常规治疗的辅助设备使用时可提供有效的消毒效果。本体外研究的目的是通过测量实验性定植于根管内粪肠球菌的杀菌效果来确定掺铒铬：钇钪石榴石（Er，Cr：YSGG）激光的杀菌效果。该激光在不同的辐照时间（30 和 60 s）和脉冲能量（75 和 25 mJ）下进行了测试。总共对 52 颗单根人牙进行了根管预备，使用旋转仪器进行预备。所有牙齿均经过消毒并接种了粪肠球菌（105 个细菌/ml）的悬浮液。将牙齿随机分为三组（第 1 组、第 2 组和第 3 组）和一组对照组。在所有组中，牙齿均用 5.25%次氯酸钠和 17%乙二胺四乙酸进行化学冲洗。第 1 组和第 2 组还分别用 75 mJ 的掺铒铬：YSGG 激光照射 30 和 60 s。第 3 组的牙齿用激光照射 60 s，能量为 25 mJ。处理样本以检测粪肠球菌的存在。对于所有组，都观察到杀菌作用。使用 75 mJ 的激光，辐照时间为 30 和 60 s，粪肠球菌的消除率分别为 92.3%和 100%。在对照组中，观察到 92.3%的降低。用 25 mJ 的激光照射 60 s 时，降低率（46.1%）较低。各组之间无统计学差异（P=0.543，Fisher 确切检验）。结果表明，在本研究中使用的设置下，掺铒铬：YSGG 激光照射具有杀菌作用。该激光的最高杀菌效果是在 60 s 的辐照时间，使用 75 mJ 的脉冲能量时观察到的。
