CmeABC外排泵对空肠弯曲菌大环内酯类和四环素耐药性的作用
Contribution of the CmeABC efflux pump to macrolide and tetracycline resistance in Campylobacter jejuni.
作者信息
Gibreel Amera, Wetsch Nicole M, Taylor Diane E
机构信息
Department of Medical Microbiology and Immunology, 1-63 Medical Sciences Building, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.
出版信息
Antimicrob Agents Chemother. 2007 Sep;51(9):3212-6. doi: 10.1128/AAC.01592-06. Epub 2007 Jul 2.
Abstract
We investigated the involvement of the CmeABC efflux pump in acquired resistance of Campylobacter jejuni to macrolides and tetracycline. Inactivation of the cmeB gene had no effect on macrolide resistance when all copies of the target gene carried an A2074C mutation. In contrast, the CmeABC pump significantly contributed to macrolide resistance when two or three copies of the 23S rRNA had an A2075G transition. Inactivation of the cmeB gene led to restoration of tetracycline susceptibility in the isolates examined. Complete susceptibility to tetracycline or macrolides, however, was not restored when phenylalanine-arginine beta-naphthylamide was used. These data confirm contribution of the CmeABC efflux pump to acquired resistance of Campylobacter jejuni to tetracycline and macrolides.
摘要
我们研究了CmeABC外排泵在空肠弯曲菌对大环内酯类和四环素获得性耐药中的作用。当靶基因的所有拷贝都携带A2074C突变时，cmeB基因的失活对大环内酯类耐药性没有影响。相反，当23S rRNA的两个或三个拷贝发生A2075G转换时，CmeABC泵对大环内酯类耐药性有显著贡献。cmeB基因的失活导致所检测分离株对四环素的敏感性恢复。然而，当使用苯丙氨酸-精氨酸β-萘酰胺时，对四环素或大环内酯类的完全敏感性并未恢复。这些数据证实了CmeABC外排泵在空肠弯曲菌对四环素和大环内酯类获得性耐药中的作用。
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