Pfeiffer Emily S, Hiasa Hiroshi
Department of Pharmacology, University of Minnesota Medical School--Twin Cities, Minneapolis, Minnesota 55455, USA.
Antimicrob Agents Chemother. 2007 Sep;51(9):3410-2. doi: 10.1128/AAC.00362-07. Epub 2007 Jul 2.
We used an assay to measure quinolone sensitivity as a shift in the position of the cleavage-religation equilibrium. This assay was found to be useful in identifying the primary target of a quinolone drug and assessing the effect of quinolone resistance-conferring mutations.
我们使用一种检测方法来测量喹诺酮敏感性,该方法是通过切割-连接平衡位置的变化来实现的。结果发现,这种检测方法对于确定喹诺酮药物的主要靶点以及评估赋予喹诺酮抗性的突变的影响很有用。