黄瓜（Cucumis sativus L.）叶绿体基因组的完整结构：其组成与比较分析。
The complete structure of the cucumber (Cucumis sativus L.) chloroplast genome: its composition and comparative analysis.
作者信息
Plader Wojciech, Yukawa Yasushi, Sugiura Masahiro, Malepszy Stefan
机构信息
Faculty of Horticulture and Landscape Architecture, Department of Plant Genetics, Breeding and Biotechnology, Warsaw Agricultural University, Nowoursynowska 159, 02-776, Warsaw, Poland.
出版信息
Cell Mol Biol Lett. 2007;12(4):584-94. doi: 10.2478/s11658-007-0029-7. Epub 2007 Jul 3.
The complete nucleotide sequence of the cucumber (C. sativus L. var. Borszczagowski) chloroplast genome has been determined. The genome is composed of 155,293 bp containing a pair of inverted repeats of 25,191 bp, which are separated by two single-copy regions, a small 18,222-bp one and a large 86,688-bp one. The chloroplast genome of cucumber contains 130 known genes, including 89 protein-coding genes, 8 ribosomal RNA genes (4 rRNA species), and 37 tRNA genes (30 tRNA species), with 18 of them located in the inverted repeat region. Of these genes, 16 contain one intron, and two genes and one ycf contain 2 introns. Twenty-one small inversions that form stem-loop structures, ranging from 18 to 49 bp, have been identified. Eight of them show similarity to those of other species, while eight seem to be cucumber specific. Detailed comparisons of ycf2 and ycf15, and the overall structure to other chloroplast genomes were performed.
已测定黄瓜（C. sativus L. var. Borszczagowski）叶绿体基因组的完整核苷酸序列。该基因组由155,293个碱基对组成，包含一对25,191个碱基对的反向重复序列，它们被两个单拷贝区域隔开，一个小的单拷贝区域为18,222个碱基对，一个大的单拷贝区域为86,688个碱基对。黄瓜的叶绿体基因组包含130个已知基因，包括89个蛋白质编码基因、8个核糖体RNA基因（4种rRNA）和37个tRNA基因（30种tRNA），其中18个位于反向重复区域。在这些基因中，16个含有一个内含子，两个基因和一个ycf含有2个内含子。已鉴定出21个形成茎环结构的小倒位，长度从18到49个碱基对不等。其中8个与其他物种的相似，而8个似乎是黄瓜特有的。对ycf2和ycf15以及与其他叶绿体基因组的整体结构进行了详细比较。
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