Riccardi Elena, Grieco Valeria, Verganti Sara, Finazzi Mario
Department of Pathology, Section of Veterinary Pathology and Avian Pathology, Veterianry Faculty, University of Milan, Via Celoria 10, 20133 Milano Italy.
J Vet Diagn Invest. 2007 Jul;19(4):431-5. doi: 10.1177/104063870701900418.
In humans and canines, the morphology of granulosa cell tumors is extremely variable and causes diagnostic difficulties. In human pathology, immunohistochemistry has been widely used for the diagnosis of granulosa cell tumors, whereas, limited studies are present in canine species. The aim of this study was to investigate the expression of cytokeratins, vimentin, and inhibin-alpha in canine normal ovaries, epithelial ovarian tumors, and granulosa cell tumors to establish an immunohistochemical panel for the differential diagnosis of ovarian tumors. Formalin-fixed, paraffin-embedded tissue sections from 4 normal ovaries, 8 granulosa cell tumors, and 6 epithelial ovarian tumors (2 adenomas and 4 adenocarcinomas) sections were obtained and stained with hematoxylin and eosin and immunohistochemically for cytokeratin AE1/AE3, cytokeratin 7, vimentin, and inhibin-alpha. In normal ovaries, cytokeratin 7, cytokeratin AE1/AE3, and vimentin were expressed in the surface epithelium. Granulosa cells were negative for cytokeratin 7 and displayed variable expression of vimentin, cytokeratin AE1/AE3, and inhibin-alpha toward follicular maturation. Granulosa cell tumors were negative for cytokeratin 7 and positive for inhibin-alpha. Conversely, ovarian epithelial cells tumors were positive for cytokeratin 7 and negative for inhibin-alpha. Both granulosa and epithelial cell tumors displayed variable expression of vimentin. Cytokeratin AE1/AE3 was expressed by all epithelial-derived tumors and 6 of 8 granulosa cell tumors. The results of this study suggest that useful immunohistochemical markers to distinguish epithelial ovarian tumors from granulosa cell tumors are cytokeratin 7 and inhibin-alpha.
在人类和犬类中，颗粒细胞瘤的形态极为多样，这给诊断带来了困难。在人类病理学中，免疫组织化学已被广泛用于颗粒细胞瘤的诊断，而在犬类中相关研究有限。本研究的目的是调查细胞角蛋白、波形蛋白和抑制素α在犬正常卵巢、卵巢上皮性肿瘤和颗粒细胞瘤中的表达，以建立用于卵巢肿瘤鉴别诊断的免疫组织化学检测方法。获取了4个正常卵巢、8个颗粒细胞瘤和6个卵巢上皮性肿瘤（2个腺瘤和4个腺癌）的福尔马林固定、石蜡包埋组织切片，进行苏木精-伊红染色以及细胞角蛋白AE1/AE3、细胞角蛋白7、波形蛋白和抑制素α的免疫组织化学染色。在正常卵巢中，细胞角蛋白7、细胞角蛋白AE1/AE3和波形蛋白在表面上皮中表达。颗粒细胞对细胞角蛋白7呈阴性，并且随着卵泡成熟，波形蛋白、细胞角蛋白AE1/AE3和抑制素α的表达存在差异。颗粒细胞瘤对细胞角蛋白7呈阴性，对抑制素α呈阳性。相反，卵巢上皮细胞肿瘤对细胞角蛋白7呈阳性，对抑制素α呈阴性。颗粒细胞瘤和上皮细胞瘤的波形蛋白表达均存在差异。所有上皮来源的肿瘤以及8个颗粒细胞瘤中的6个表达细胞角蛋白AE1/AE3。本研究结果表明，用于区分卵巢上皮性肿瘤和颗粒细胞瘤的有用免疫组织化学标志物是细胞角蛋白7和抑制素α。
