Voss S C, Thevis M, Schinkothe T, Schänzer W
Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Cologne, Germany.
Int J Sports Med. 2007 Aug;28(8):633-7. doi: 10.1055/s-2007-965076. Epub 2007 Jul 5.
The aim of the present study was to improve and validate a flow cytometric method for the detection of homologous blood transfusion in doping control analysis. A panel of eight different primary antibodies and two different phycoerythrin-conjugated secondary antibodies was used for the detection of different blood populations. The flow cytometer used in this study was the BD FACSArray instrument. Mixed red blood cell populations were prepared from phenotype known donors. Linearity, specificity, recovery, precision, robustness and interday-precision were tested for every primary antibody used in the presented assay. The technique of signal amplification was utilized for an improved separation of antigens with weak or heterozygous expression to improve the interpretation of histograms. The resulting method allowed to clearly identify mixed red blood cell populations in homologous blood transfusion samples containing 0.3 - 2.0 % of donor blood.
本研究的目的是改进和验证一种用于兴奋剂检测分析中同源输血检测的流式细胞术方法。使用一组八种不同的一抗和两种不同的藻红蛋白偶联二抗来检测不同的血细胞群体。本研究中使用的流式细胞仪是BD FACSArray仪器。混合红细胞群体由已知表型的供体制备。对本试验中使用的每种一抗进行线性、特异性、回收率、精密度、稳健性和日间精密度测试。利用信号放大技术改进对表达较弱或杂合的抗原的分离，以改善直方图的解读。所得方法能够清晰识别同源输血样本中含0.3 - 2.0%供体血液的混合红细胞群体。
