Vautor E, Magnone V, Rios G, Le Brigand K, Bergonier D, Lina G, Meugnier H, Barbry P, Thiéry R, Pépin M
Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Unité Pathologie des Petits Ruminants, Sophia-Antipolis, France.
Vet Microbiol. 2009 Jan 1;133(1-2):105-14. doi: 10.1016/j.vetmic.2008.06.006. Epub 2008 Jun 8.
Staphylococcus aureus is recognized worldwide as a major pathogen causing clinical or subclinical intramammary infections in lactating sheep, goats and cows. The present study was carried out to compare 65 S. aureus isolates mainly obtained from nasal carriage and subclinical mastitis in dairy sheep and 43 isolates obtained from subclinical mastitis from 22 goats and 21 cows. A DNA microarray, containing probes against 190 true or putative virulence factors, was used to detect the presence of the virulence genes. Their presence/absence was independently assessed by PCR for the genes of interest. Sheep isolates obtained from the nostrils or the udders did not show any significant tissue specific virulence factor. The dominant pulse-field electrophoresis profile (OV/OV'), associated with spa clonal complex spa-CC 1773, matched mainly with the agr group III and was only found in ovine and caprine isolates. This clone was more specifically characterized by the prevalence of the following virulence genes: lpl4, ssl6, bsaA1, bsaB, bsaP, SAV0812. Moreover, seven virulence-associated genes (lpl1, sel, sec, tst, lukF-PV-like component, lukM, SAV0876) were associated with isolates from small ruminants, while the egc cluster, fhuD1, abiF and SAV2496 with bovine isolates. This genomic study suggests the existence of lineage- and host-specific genes leading to the development of host-specific pathogenic traits of S. aureus isolates.
金黄色葡萄球菌是一种在全球范围内被公认为可导致泌乳绵羊、山羊和奶牛发生临床或亚临床乳房内感染的主要病原体。本研究旨在比较主要从奶羊鼻腔携带菌和亚临床乳腺炎中分离得到的65株金黄色葡萄球菌，以及从22只山羊和21头奶牛的亚临床乳腺炎中分离得到的43株菌株。使用包含针对190种真实或假定毒力因子的探针的DNA微阵列来检测毒力基因的存在。通过针对感兴趣基因的PCR独立评估它们的存在与否。从鼻孔或乳房获得的绵羊分离株未显示出任何明显的组织特异性毒力因子。与spa克隆复合体spa-CC 1773相关的主要脉冲场凝胶电泳图谱（OV/OV'），主要与agr III组匹配，仅在绵羊和山羊分离株中发现。该克隆的更具体特征是以下毒力基因的流行：lpl4、ssl6、bsaA1、bsaB、bsaP、SAV0812。此外，七个毒力相关基因（lpl1、sel、sec、tst、lukF-PV样成分、lukM、SAV0876）与小反刍动物的分离株相关，而egc簇、fhuD1、abiF和SAV2496与牛的分离株相关。这项基因组研究表明存在谱系特异性和宿主特异性基因，导致金黄色葡萄球菌分离株出现宿主特异性致病特征。
