用于粪便和水样中隐孢子虫卵囊灵敏检测的环介导等温扩增方法的建立及初步评估
Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of cryptosporidium oocysts in fecal and water samples.
作者信息
Karanis Panagiotis, Thekisoe Oriel, Kiouptsi Klytaimnistra, Ongerth Jerry, Igarashi Ikuo, Inoue Noborou
机构信息
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Hokkaido 080-8555, Japan.
出版信息
Appl Environ Microbiol. 2007 Sep;73(17):5660-2. doi: 10.1128/AEM.01152-07. Epub 2007 Jul 6.
Abstract
A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence.
摘要
开发并评估了一种用于检测环境和粪便样本中隐孢子虫的环介导等温扩增（LAMP）方法。这是首次证明LAMP可用于检测隐孢子虫。由于其特异性和简便性，该方法可能成为隐孢子虫存在情况流行病学研究的有用诊断工具。
相似文献
1
Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of cryptosporidium oocysts in fecal and water samples.用于粪便和水样中隐孢子虫卵囊灵敏检测的环介导等温扩增方法的建立及初步评估
Appl Environ Microbiol. 2007 Sep;73(17):5660-2. doi: 10.1128/AEM.01152-07. Epub 2007 Jul 6.
2
Sensitive and specific detection of Cryptosporidium species in PCR-negative samples by loop-mediated isothermal DNA amplification and confirmation of generated LAMP products by sequencing.通过环介导等温DNA扩增对PCR阴性样本中的隐孢子虫物种进行灵敏且特异的检测，并通过测序确认所产生的环介导等温扩增产物。
Vet Parasitol. 2008 Nov 25;158(1-2):11-22. doi: 10.1016/j.vetpar.2008.09.012. Epub 2008 Sep 11.
3
Recombinase polymerase amplification (RPA) combined with lateral flow (LF) strip for equipment-free detection of Cryptosporidium spp. oocysts in dairy cattle feces.重组酶聚合酶扩增（RPA）结合侧向流动（LF）条带用于在无设备情况下检测奶牛粪便中的隐孢子虫属卵囊。
Parasitol Res. 2016 Sep;115(9):3551-5. doi: 10.1007/s00436-016-5120-4. Epub 2016 May 13.
4
Development and evaluation of a reverse transcription-loop-mediated isothermal amplification assay for rapid and high-sensitive detection of Cryptosporidium in water samples.用于快速高灵敏检测水样中隐孢子虫的逆转录环介导等温扩增检测方法的建立与评估
Water Sci Technol. 2009;60(8):2167-72. doi: 10.2166/wst.2009.599.
5
Investigations and comparative detection of Cryptosporidium species by microscopy, nested PCR and LAMP in water supplies of Ordu, Middle Black Sea, Turkey.土耳其中黑海地区奥尔杜市供水系统中隐孢子虫种类的显微镜检查、巢式PCR及环介导等温扩增检测与比较
Ann Trop Med Parasitol. 2011 Dec;105(8):607-15. doi: 10.1179/2047773211Y.0000000011.
6
The role of aquatic birds in the environmental dissemination of human pathogenic Giardia duodenalis cysts and Cryptosporidium oocysts in Hungary.水鸟在匈牙利人类致病性十二指肠贾第虫包囊和隐孢子虫卵囊的环境传播中的作用。
Parasitol Int. 2009 Sep;58(3):227-31. doi: 10.1016/j.parint.2009.05.004. Epub 2009 May 13.
7
Rapid and sensitive detection of single cryptosporidium oocysts from archived glass slides.从存档载玻片上快速灵敏地检测单个隐孢子虫卵囊。
J Clin Microbiol. 2006 Sep;44(9):3285-91. doi: 10.1128/JCM.00541-06.
8
Combination of ARAD microfibre filtration and LAMP methodology for simple, rapid and cost-effective detection of human pathogenic Giardia duodenalis and Cryptosporidium spp. in drinking water.联合使用 ARAD 微纤维过滤和 LAMP 方法，可简单、快速且经济高效地检测饮用水中的人体致病原生动物贾第虫属和隐孢子虫属。
Lett Appl Microbiol. 2010 Jan;50(1):82-8. doi: 10.1111/j.1472-765X.2009.02758.x.
9
10
The sensitivity of PCR detection of Cryptosporidium oocysts in fecal samples using two DNA extraction methods.使用两种DNA提取方法对粪便样本中隐孢子虫卵囊进行PCR检测的灵敏度。
Mol Diagn. 2003;7(3-4):147-53. doi: 10.1007/BF03260031.
引用本文的文献
1
Development of a "Rapid-Crypto Colorimetric LAMP Test" to Detect Cryptosporidiosis in Feces of Newborns Calves.快速Crypto 比色环介导等温扩增检测技术在新生犊牛粪便中隐孢子虫病的检测
Acta Parasitol. 2024 Mar;69(1):691-699. doi: 10.1007/s11686-023-00791-x. Epub 2024 Feb 15.
2
Rapid detection of spp. in diarrheic cattle feces by isothermal recombinase polymerase amplification assays.通过等温重组酶聚合酶扩增分析法快速检测腹泻牛粪便中的 spp.
Heliyon. 2023 Oct 7;9(10):e20794. doi: 10.1016/j.heliyon.2023.e20794. eCollection 2023 Oct.
3
Diagnostic Methods of Common Intestinal Protozoa: Current and Future Immunological and Molecular Methods.常见肠道原生动物的诊断方法：当前及未来的免疫学和分子学方法
Trop Med Infect Dis. 2022 Sep 21;7(10):253. doi: 10.3390/tropicalmed7100253.
4
Development and Preliminary Evaluation of a Nanoparticle-Assisted PCR Assay for the Detection of in Calves.用于检测犊牛中[具体物质未给出]的纳米颗粒辅助聚合酶链反应检测方法的开发与初步评估。
Animals (Basel). 2022 Aug 1;12(15):1953. doi: 10.3390/ani12151953.
5
Detection of SARS-CoV-2 in different human biofluids using the loop-mediated isothermal amplification assay: A prospective diagnostic study in Fortaleza, Brazil.使用环介导等温扩增法检测不同人体生物流体中的新型冠状病毒2：巴西福塔雷萨的一项前瞻性诊断研究
J Med Virol. 2022 Sep;94(9):4170-4180. doi: 10.1002/jmv.27842. Epub 2022 May 20.
6
Development of a New LAMP Assay for the Detection of DNA (Copro-LAMPAc) in Dog Fecal Samples.用于检测犬粪便样本中DNA（粪便环介导等温扩增法）的新型环介导等温扩增检测方法的开发。
Front Vet Sci. 2021 Nov 12;8:770508. doi: 10.3389/fvets.2021.770508. eCollection 2021.
7
Omics-Based Analytical Approaches for Assessing Chicken Species and Breeds in Food Authentication.基于组学的分析方法在食品真实性鉴别中的鸡种和品种鉴定。
Molecules. 2021 Oct 28;26(21):6502. doi: 10.3390/molecules26216502.
8
Optimization and validation of a loop-mediated isothermal amplification (LAMP) assay for detection of in leafy greens.用于检测绿叶蔬菜中[具体物质未给出]的环介导等温扩增（LAMP）检测方法的优化与验证
Food Waterborne Parasitol. 2021 Apr 26;23:e00123. doi: 10.1016/j.fawpar.2021.e00123. eCollection 2021 Jun.
9
Development of a Loop-mediated isothermal amplification (LAMP) technique for specific and early detection of Mycobacterium leprae in clinical samples.建立环介导等温扩增（LAMP）技术，用于临床样本中麻风分枝杆菌的特异性和早期检测。
Sci Rep. 2021 May 10;11(1):9859. doi: 10.1038/s41598-021-89304-2.
10
An innovative and user-friendly smartphone-assisted molecular diagnostic approach for rapid detection of canine vector-borne diseases.一种创新且用户友好的智能手机辅助分子诊断方法，用于快速检测犬类媒介传染病。
Parasitol Res. 2021 May;120(5):1799-1809. doi: 10.1007/s00436-021-07077-z. Epub 2021 Mar 2.
本文引用的文献
1
Waterborne transmission of protozoan parasites: a worldwide review of outbreaks and lessons learnt.原生动物寄生虫的水传播：全球疫情回顾及经验教训
J Water Health. 2007 Mar;5(1):1-38. doi: 10.2166/wh.2006.002.
2
Evaluation and applicability of a purification method coupled with nested PCR for the detection of Toxoplasma oocysts in water.一种用于检测水中弓形虫卵囊的纯化方法结合巢式PCR的评估及适用性
Lett Appl Microbiol. 2006 Nov;43(5):475-81. doi: 10.1111/j.1472-765X.2006.02008.x.
3
Development of loop-mediated isothermal amplification (LAMP) method for diagnosis of equine piroplasmosis.用于诊断马焦虫病的环介导等温扩增（LAMP）方法的开发。
Vet Parasitol. 2007 Jan 31;143(2):155-60. doi: 10.1016/j.vetpar.2006.08.014. Epub 2006 Sep 14.
4
Occurrence of Giardia and Cryptosporidium in water supplies of Russia and Bulgaria.俄罗斯和保加利亚供水系统中贾第虫和隐孢子虫的出现情况。
Environ Res. 2006 Nov;102(3):260-71. doi: 10.1016/j.envres.2006.05.005. Epub 2006 Jun 14.
5
The zoonotic transmission of Giardia and Cryptosporidium.贾第虫和隐孢子虫的人畜共患传播。
Int J Parasitol. 2005 Oct;35(11-12):1181-90. doi: 10.1016/j.ijpara.2005.07.009.
6
Lamp for tadpoles.蝌蚪灯。
Nat Methods. 2005 Sep;2(9):635; author reply 635-6. doi: 10.1038/nmeth0905-635a.
7
Evaluation of loop-mediated isothermal amplification (LAMP), PCR and parasitological tests for detection of Trypanosoma evansi in experimentally infected pigs.评价环介导等温扩增技术（LAMP）、聚合酶链反应（PCR）和寄生虫学检测方法用于检测实验感染猪体内的伊氏锥虫。
Vet Parasitol. 2005 Jun 30;130(3-4):327-30. doi: 10.1016/j.vetpar.2005.04.019.
8
Molecular evidence of infections with Babesia gibsoni parasites in Japan and evaluation of the diagnostic potential of a loop-mediated isothermal amplification method.日本吉氏巴贝斯虫感染的分子证据及环介导等温扩增法诊断潜力的评估
J Clin Microbiol. 2004 Jun;42(6):2465-9. doi: 10.1128/JCM.42.6.2465-2469.2004.
9
Loop-mediated isothermal amplification for detection of African trypanosomes.用于检测非洲锥虫的环介导等温扩增技术。
J Clin Microbiol. 2003 Dec;41(12):5517-24. doi: 10.1128/JCM.41.12.5517-5524.2003.
10
Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation.通过焦磷酸镁形成产生的浊度检测环介导等温扩增反应。
Biochem Biophys Res Commun. 2001 Nov 23;289(1):150-4. doi: 10.1006/bbrc.2001.5921.
Zotero 插件