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Waterborne transmission of protozoan parasites: a worldwide review of outbreaks and lessons learnt.原生动物寄生虫的水传播：全球疫情回顾及经验教训
J Water Health. 2007 Mar;5(1):1-38. doi: 10.2166/wh.2006.002.
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Evaluation and applicability of a purification method coupled with nested PCR for the detection of Toxoplasma oocysts in water.一种用于检测水中弓形虫卵囊的纯化方法结合巢式PCR的评估及适用性
Lett Appl Microbiol. 2006 Nov;43(5):475-81. doi: 10.1111/j.1472-765X.2006.02008.x.
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Development of loop-mediated isothermal amplification (LAMP) method for diagnosis of equine piroplasmosis.用于诊断马焦虫病的环介导等温扩增（LAMP）方法的开发。
Vet Parasitol. 2007 Jan 31;143(2):155-60. doi: 10.1016/j.vetpar.2006.08.014. Epub 2006 Sep 14.
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Occurrence of Giardia and Cryptosporidium in water supplies of Russia and Bulgaria.俄罗斯和保加利亚供水系统中贾第虫和隐孢子虫的出现情况。
Environ Res. 2006 Nov;102(3):260-71. doi: 10.1016/j.envres.2006.05.005. Epub 2006 Jun 14.
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The zoonotic transmission of Giardia and Cryptosporidium.贾第虫和隐孢子虫的人畜共患传播。
Int J Parasitol. 2005 Oct;35(11-12):1181-90. doi: 10.1016/j.ijpara.2005.07.009.
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Lamp for tadpoles.蝌蚪灯。
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Evaluation of loop-mediated isothermal amplification (LAMP), PCR and parasitological tests for detection of Trypanosoma evansi in experimentally infected pigs.评价环介导等温扩增技术（LAMP）、聚合酶链反应（PCR）和寄生虫学检测方法用于检测实验感染猪体内的伊氏锥虫。
Vet Parasitol. 2005 Jun 30;130(3-4):327-30. doi: 10.1016/j.vetpar.2005.04.019.
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Molecular evidence of infections with Babesia gibsoni parasites in Japan and evaluation of the diagnostic potential of a loop-mediated isothermal amplification method.日本吉氏巴贝斯虫感染的分子证据及环介导等温扩增法诊断潜力的评估
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Loop-mediated isothermal amplification for detection of African trypanosomes.用于检测非洲锥虫的环介导等温扩增技术。
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Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation.通过焦磷酸镁形成产生的浊度检测环介导等温扩增反应。
Biochem Biophys Res Commun. 2001 Nov 23;289(1):150-4. doi: 10.1006/bbrc.2001.5921.

用于粪便和水样中隐孢子虫卵囊灵敏检测的环介导等温扩增方法的建立及初步评估

Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of cryptosporidium oocysts in fecal and water samples.

作者信息

Karanis Panagiotis, Thekisoe Oriel, Kiouptsi Klytaimnistra, Ongerth Jerry, Igarashi Ikuo, Inoue Noborou

机构信息

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Hokkaido 080-8555, Japan.

出版信息

Appl Environ Microbiol. 2007 Sep;73(17):5660-2. doi: 10.1128/AEM.01152-07. Epub 2007 Jul 6.

DOI:10.1128/AEM.01152-07
PMID:17616628
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2042060/
Abstract

A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence.

摘要

开发并评估了一种用于检测环境和粪便样本中隐孢子虫的环介导等温扩增（LAMP）方法。这是首次证明LAMP可用于检测隐孢子虫。由于其特异性和简便性，该方法可能成为隐孢子虫存在情况流行病学研究的有用诊断工具。