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Waterborne transmission of protozoan parasites: review of worldwide outbreaks - an update 2004-2010.水传播原生动物寄生虫：全球暴发回顾 - 2004-2010 年更新。
Water Res. 2011 Dec 15;45(20):6603-14. doi: 10.1016/j.watres.2011.10.013. Epub 2011 Oct 20.
2
LAMP--a powerful and flexible tool for monitoring microbial pathogens.环介导等温扩增技术——一种用于监测微生物病原体的强大且灵活的工具。
Trends Parasitol. 2009 Nov;25(11):498-9. doi: 10.1016/j.pt.2009.07.010. Epub 2009 Sep 4.
3
Rapid identification of Giardia duodenalis by loop-mediated isothermal amplification (LAMP) from faecal and environmental samples and comparative findings by PCR and real-time PCR methods.通过环介导等温扩增技术（LAMP）从粪便和环境样本中快速鉴定十二指肠贾第虫，并与聚合酶链反应（PCR）和实时荧光定量PCR方法的比较结果
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4
Sensitive and specific detection of Cryptosporidium species in PCR-negative samples by loop-mediated isothermal DNA amplification and confirmation of generated LAMP products by sequencing.通过环介导等温DNA扩增对PCR阴性样本中的隐孢子虫物种进行灵敏且特异的检测，并通过测序确认所产生的环介导等温扩增产物。
Vet Parasitol. 2008 Nov 25;158(1-2):11-22. doi: 10.1016/j.vetpar.2008.09.012. Epub 2008 Sep 11.
5
Evaluation of loop-mediated isothermal amplification for detection of Toxoplasma gondii in water samples and comparative findings by polymerase chain reaction and immunofluorescence test (IFT).环介导等温扩增法检测水样中弓形虫的评估及与聚合酶链反应和免疫荧光试验（IFT）的比较结果
Diagn Microbiol Infect Dis. 2008 Dec;62(4):357-65. doi: 10.1016/j.diagmicrobio.2008.07.009. Epub 2008 Aug 20.
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Detection and characterisation of Giardia and Cryptosporidium in Hungarian raw, surface and sewage water samples by IFT, PCR and sequence analysis of the SSUrRNA and GDH genes.通过IFT、PCR以及对SSUrRNA和GDH基因进行序列分析，检测和鉴定匈牙利原水、地表水及污水样本中的贾第虫和隐孢子虫。
Int J Hyg Environ Health. 2008 Oct;211(5-6):524-33. doi: 10.1016/j.ijheh.2008.04.004. Epub 2008 Jun 11.
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Cryptosporidium and Giardia as foodborne zoonoses.隐孢子虫病和贾第虫病作为食源性人畜共患病。
Vet Parasitol. 2007 Oct 21;149(1-2):29-40. doi: 10.1016/j.vetpar.2007.07.015. Epub 2007 Aug 28.
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Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of cryptosporidium oocysts in fecal and water samples.用于粪便和水样中隐孢子虫卵囊灵敏检测的环介导等温扩增方法的建立及初步评估
Appl Environ Microbiol. 2007 Sep;73(17):5660-2. doi: 10.1128/AEM.01152-07. Epub 2007 Jul 6.
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Waterborne transmission of protozoan parasites: a worldwide review of outbreaks and lessons learnt.原生动物寄生虫的水传播：全球疫情回顾及经验教训
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10
Evaluation and applicability of a purification method coupled with nested PCR for the detection of Toxoplasma oocysts in water.一种用于检测水中弓形虫卵囊的纯化方法结合巢式PCR的评估及适用性
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土耳其中黑海地区奥尔杜市供水系统中隐孢子虫种类的显微镜检查、巢式PCR及环介导等温扩增检测与比较

Investigations and comparative detection of Cryptosporidium species by microscopy, nested PCR and LAMP in water supplies of Ordu, Middle Black Sea, Turkey.

作者信息

Koloren Z, Sotiriadou I, Karanis P

机构信息

Faculty of Arts and Sciences, University of Ordu, Ordu, Turkey.

出版信息

Ann Trop Med Parasitol. 2011 Dec;105(8):607-15. doi: 10.1179/2047773211Y.0000000011.

DOI:10.1179/2047773211Y.0000000011
PMID:22325820
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4089801/
Abstract

A total of 70 water samples, including tap, river, fountain and well water were collected in the Ordu province, Middle Black Sea, Turkey and investigated for the detection of Cryptosporidium oocysts. The samples were directly screened microscopically for Cryptosporidium oocysts' detection by immunofluorescence test and subsequently DNA was extracted for the molecular detection by loop-mediated isothermal amplification (LAMP) and nested polymerase chain reaction (PCR). Eighteen out of the 70 (25·7%) water samples were found positive for Cryptosporidium spp. by immunofluorescence test and 19 (27·1%) were found positive by LAMP. Nested PCR products were not generated in any of the investigated water samples. A total of 16 randomly selected pellets were spiked with 10 Cryptosporidium oocysts to test the efficiency of the applied method. All the samples were found positive by LAMP for the presence of Cryptosporidium DNA, while the nested PCR assay was positive in only seven (43·75%) out of the 16 examined spiked samples. This is the first report on the occurrence of Cryptosporidium species in environmental and drinking water supplies in the Black Sea area.

摘要

在土耳其中黑海地区的奥尔杜省共采集了70份水样，包括自来水、河水、喷泉和井水，并对隐孢子虫卵囊的检测进行了调查。通过免疫荧光试验直接对样本进行显微镜筛查以检测隐孢子虫卵囊，随后提取DNA，采用环介导等温扩增技术（LAMP）和巢式聚合酶链反应（PCR）进行分子检测。70份水样中有18份（25.7%）通过免疫荧光试验检测出隐孢子虫属呈阳性，19份（27.1%）通过LAMP检测呈阳性。在所调查的任何水样中均未产生巢式PCR产物。总共随机选择16个沉淀样本，加入10个隐孢子虫卵囊以测试所应用方法的效率。所有样本通过LAMP检测均发现隐孢子虫DNA呈阳性，而在16个检测的加标样本中，巢式PCR检测仅7个（43.75%）呈阳性。这是关于黑海地区环境和饮用水供应中隐孢子虫种类存在情况的首份报告。