美国女性血浆和红细胞脂肪酸含量作为脂肪酸摄入量生物标志物的比较。

Comparison between plasma and erythrocyte fatty acid content as biomarkers of fatty acid intake in US women.

作者信息

Sun Qi, Ma Jing, Campos Hannia, Hankinson Susan E, Hu Frank B

机构信息

Department of Nutrition, Harvard School of Public Health, Boston, MA 02115, USA.

出版信息

Am J Clin Nutr. 2007 Jul;86(1):74-81. doi: 10.1093/ajcn/86.1.74.

DOI:10.1093/ajcn/86.1.74

PMID:17616765

Abstract

BACKGROUND

Erythrocyte fatty acids may be superior to plasma fatty acids for reflecting long-term fatty acid intake because of less sensitivity to recent intake and a slower turnover rate.

OBJECTIVE

The objective was to compare the fatty acid content of erythrocytes with that of plasma with respect to their abilities to reflect usual fatty acid intake.

DESIGN

Fatty acids in plasma and erythrocytes were measured by capillary gas-liquid chromatography in 306 US women aged 43-69 y. Fatty acid intake was assessed with a food-frequency questionnaire, which was validated for measuring intakes of various fatty acids.

RESULTS

Docosahexaenoic acid (DHA, 22:6n-3) in erythrocytes and plasma provided the strongest correlations with its intake, but erythrocyte DHA concentrations [Spearman's partial correlation coefficient (r(s))=0.56] were better than plasma DHA concentrations (r(s)=0.48) as a biomarker. Total trans fatty acids (r(s)=0.43) and total 18:1 trans isomers (r(s)=0.42) in erythrocytes were also more strongly correlated with intake than were those in plasma (r(s)=0.30 and r(s)=0.29, respectively). Moderate correlations were observed for linoleic acid (18:2n-6; erythrocytes, r(s)=0.24; plasma, r(s)=0.25), alpha-linolenic acid (18:3n-3; erythrocytes, r(s)=0.18; plasma, r(s)=0.23), and eicosapentaenoic acid (20:5 n-3; erythrocytes, r(s)=0.38; plasma, r(s)=0.21). For polyunsaturated and trans fatty acids, correlations between intakes and biomarkers improved moderately when average intakes over previous years were used.

CONCLUSION

Erythrocyte n-3 fatty acids of marine origin and trans fatty acid content are suitable biomarkers for long-term intake.

摘要

背景

红细胞脂肪酸在反映长期脂肪酸摄入量方面可能优于血浆脂肪酸，因为其对近期摄入量的敏感性较低且周转率较慢。

目的

比较红细胞和血浆的脂肪酸含量在反映通常脂肪酸摄入量方面的能力。

设计

采用毛细管气液色谱法测定了306名年龄在43 - 69岁的美国女性血浆和红细胞中的脂肪酸。通过食物频率问卷评估脂肪酸摄入量，该问卷已被验证可用于测量各种脂肪酸的摄入量。

结果

红细胞和血浆中的二十二碳六烯酸（DHA，22:6n - 3）与其摄入量的相关性最强，但作为生物标志物，红细胞DHA浓度[斯皮尔曼偏相关系数（r(s)）=0.56]优于血浆DHA浓度（r(s)=0.48）。红细胞中的总反式脂肪酸（r(s)=0.43）和总18:1反式异构体（r(s)=0.42）与摄入量的相关性也比血浆中的更强（分别为r(s)=0.30和r(s)=0.29）。亚油酸（18:2n - 6；红细胞，r(s)=0.24；血浆，r(s)=0.25）、α-亚麻酸（18:3n - 3；红细胞，r(s)=0.18；血浆，r(s)=0.23）和二十碳五烯酸（20:5 n - 3；红细胞，r(s)=0.38；血浆，r(s)=0.21）的相关性为中等。对于多不饱和脂肪酸和反式脂肪酸，当使用前几年的平均摄入量时，摄入量与生物标志物之间的相关性适度提高。