Han N, Hoover C I, Winkler J R, Ng C Y, Armitage G C
Department of Periodontology, School of Stomatology, Beijing Medical University, China.
J Clin Microbiol. 1991 Aug;29(8):1574-8. doi: 10.1128/jcm.29.8.1574-1578.1991.
To evaluate its utility in discriminating different strains, restriction endonuclease analysis was applied to 12 strains of Actinobacillus actinomycetemcomitans (3 serotype a, 5 serotype b, and 4 serotype c strains). DNA isolated from each strain was digested by 12 different restriction endonucleases, and the electrophoretic banding patterns of the resulting DNA fragments were compared. The DNA fragment patterns produced by SalI, XhoI, and XbaI for the 12 A. actinomycetemcomitans strains were simple (less than 30 bands) and allowed us to recognize easily 10 distinct genomic clonal types. The three serotype a strains exhibited distinctly different clonal types from one another, the five serotype b strains exhibited an additional four distinct clonal types, and the four serotype c strains showed another three different clonal types. The other endonucleases tested were less useful in typing A. actinomycetemcomitans. We conclude that restriction endonuclease analysis is a powerful tool for typing and discerning genetic heterogeneity and homogeneity among A. actinomycetemcomitans strains. It should, therefore, be very useful for epidemiologic studies.
为评估其在区分不同菌株方面的效用，对12株伴放线放线杆菌（3株a血清型、5株b血清型和4株c血清型菌株）进行了限制性内切酶分析。从每株菌株中分离的DNA用12种不同的限制性内切酶进行消化，并比较所得DNA片段的电泳条带模式。SalI、XhoI和XbaI对12株伴放线放线杆菌产生的DNA片段模式很简单（少于30条带），使我们能够轻松识别出10种不同的基因组克隆类型。3株a血清型菌株彼此表现出明显不同的克隆类型，5株b血清型菌株表现出另外4种不同的克隆类型，4株c血清型菌株表现出另外3种不同的克隆类型。所测试的其他内切酶在伴放线放线杆菌分型方面用处较小。我们得出结论，限制性内切酶分析是伴放线放线杆菌菌株分型以及识别遗传异质性和同质性的有力工具。因此，它对流行病学研究应该非常有用。
