Zambon J J, DeLuca C, Slots J, Genco R J
Infect Immun. 1983 Apr;40(1):205-12. doi: 10.1128/iai.40.1.205-212.1983.
The promyelocytic HL-60 cell line was examined for susceptibility to leukotoxin from Actinobacillus actinomycetemcomitans. Strains of A. actinomycetemcomitans which caused lysis of human peripheral blood polymorphonuclear leukocytes also lysed HL-60 cells as determined by release of intracellular lactate dehydrogenase. The killing of HL-60 cells by A. actinomycetemcomitans was dose dependent and temperature dependent, reached maximal levels after 45 min of incubation, and was inhibited by rabbit antisera to A. actinomycetemcomitans. Of 100 oral isolates of A. actinomycetemcomitans from 55 subjects, 16% from 11 healthy subjects, 43% from 13 adult periodontitis patients, 75% from 4 insulin-dependent diabetics, 66% from 2 generalized juvenile periodontitis patients, and 55% from 25 localized juvenile periodontitis patients produced leukotoxin. The same subject could harbor both leukotoxin-producing and -nonproducing isolates. The significantly higher proportion of leukotoxin-producing isolates in the disease groups compared with the healthy group is consistent with the hypothesis that leukotoxin from A. actinomycetemcomitans is an important virulence factor in the pathogenesis of certain forms of periodontal disease.
检测了早幼粒细胞HL-60细胞系对伴放线放线杆菌白细胞毒素的敏感性。通过细胞内乳酸脱氢酶的释放测定，能导致人外周血多形核白细胞溶解的伴放线放线杆菌菌株也能溶解HL-60细胞。伴放线放线杆菌对HL-60细胞的杀伤作用具有剂量依赖性和温度依赖性，孵育45分钟后达到最大水平，并且被抗伴放线放线杆菌兔抗血清所抑制。在来自55名受试者的100株口腔伴放线放线杆菌分离株中，11名健康受试者的分离株中有16%产生白细胞毒素，13名成人牙周炎患者的分离株中有43%产生白细胞毒素，4名胰岛素依赖型糖尿病患者的分离株中有75%产生白细胞毒素，2名广泛型青少年牙周炎患者的分离株中有66%产生白细胞毒素，25名局限型青少年牙周炎患者的分离株中有55%产生白细胞毒素。同一受试者可能同时携带产白细胞毒素和不产白细胞毒素的分离株。与健康组相比，疾病组中产白细胞毒素分离株的比例显著更高，这与伴放线放线杆菌白细胞毒素是某些形式牙周病发病机制中重要毒力因子的假设一致。
