Kappe R, Müller J
Mycology Section, Institute for Medical Microbiology and Hygiene, University of Freiburg, Federal Republic of Germany.
J Clin Microbiol. 1991 Aug;29(8):1665-9. doi: 10.1128/jcm.29.8.1665-1669.1991.
Clearances of mannan antigen from Candida albicans and glucuronoxylomannan antigen from Cryptococcus neoformans were examined in nonimmune rabbits by using self-prepared latex agglutination tests. Injected intravenously, 20 mg of Candida mannan antigen was cleared from the serum with a half-life of approximately 2 h. In contrast, 20 micrograms of Cryptococcus glucuronoxylomannan antigen had a half-life in serum of approximately 24 h. At the latest, 9 h after injection, both antigens were no longer detectable without pretreatment of serum samples with protease and heating to 100 degrees C, thus indicating rapid binding by serum proteins other than immunoglobulins. Candida mannan antigen clearance was also examined in nonimmune mice after intravenous injection of (i) 200 micrograms of Candida mannan antigen, which accumulated in the liver and spleen and persisted for 97 days; (ii) 2 x 10(7) ethanol-killed Candida blastospores, which was accompanied by rapid clearance of mannan from the blood but accumulation of mannan in the liver and spleen and slow clearance from these organs; (iii) 6 x 10(6) viable C. albicans cells (lethal infection), which resulted in a rapid decrease of Candida CFU in the blood, liver, and spleen during the first 8 h, after which blood cultures were negative on day 2 and viable Candida burdens in the liver and spleen persisted at 10(5) CFU/g, whereas Candida mannan antigen continued to circulate in the bloodstream and accumulated in the liver and spleen.
通过使用自行制备的乳胶凝集试验，在非免疫兔中检测了白色念珠菌甘露聚糖抗原和新型隐球菌葡糖醛酸木聚糖甘露聚糖抗原的清除情况。静脉注射20mg白色念珠菌甘露聚糖抗原后，血清中的该抗原以约2小时的半衰期被清除。相比之下，20μg新型隐球菌葡糖醛酸木聚糖甘露聚糖抗原在血清中的半衰期约为24小时。最迟在注射后9小时，若不先用蛋白酶预处理血清样品并加热至100℃，两种抗原均无法检测到，这表明它们被非免疫球蛋白的血清蛋白快速结合。在非免疫小鼠静脉注射以下物质后，也检测了白色念珠菌甘露聚糖抗原的清除情况：(i)200μg白色念珠菌甘露聚糖抗原，其在肝脏和脾脏中蓄积并持续97天；(ii)2×10⁷个经乙醇杀死的白色念珠菌芽生孢子，其伴随甘露聚糖从血液中的快速清除，但甘露聚糖在肝脏和脾脏中蓄积且从这些器官中缓慢清除；(iii)6×10⁶个白色念珠菌活细胞（致死性感染），这导致在最初8小时内血液、肝脏和脾脏中的白色念珠菌CFU迅速减少，此后第2天血培养呈阴性，肝脏和脾脏中的白色念珠菌活菌载量持续维持在10⁵CFU/g，而白色念珠菌甘露聚糖抗原继续在血液中循环并在肝脏和脾脏中蓄积。
