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用于检测临床样本中结核分枝杆菌的巢式聚合酶链反应

Nested polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples.

作者信息

Miyazaki Y, Koga H, Kohno S, Kaku M

机构信息

Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.

出版信息

J Clin Microbiol. 1993 Aug;31(8):2228-32. doi: 10.1128/jcm.31.8.2228-2232.1993.

DOI:10.1128/jcm.31.8.2228-2232.1993
PMID:8370757
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC265731/
Abstract

The nested polymerase chain reaction technique was compared with the conventional smear and culture methods for detection of Mycobacterium tuberculosis. The nested polymerase chain reaction used in this study showed excellent specificity, sensitivity, and agreement with the conventional methods for 417 clinical samples, indicating a contribution to the rapid diagnosis of mycobacterial infectious diseases.

摘要

将巢式聚合酶链反应技术与传统涂片和培养方法用于检测结核分枝杆菌进行比较。本研究中使用的巢式聚合酶链反应对417份临床样本显示出优异的特异性、敏感性，且与传统方法结果一致，表明其对分枝杆菌感染性疾病的快速诊断有贡献。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51b9/265731/043aff6cf1ad/jcm00020-0283-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51b9/265731/2379e6cc1fb7/jcm00020-0282-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51b9/265731/3d6d8b73c7cf/jcm00020-0282-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51b9/265731/639ef531e4d6/jcm00020-0282-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51b9/265731/330dd269d9e4/jcm00020-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51b9/265731/043aff6cf1ad/jcm00020-0283-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51b9/265731/2379e6cc1fb7/jcm00020-0282-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51b9/265731/3d6d8b73c7cf/jcm00020-0282-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51b9/265731/639ef531e4d6/jcm00020-0282-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51b9/265731/330dd269d9e4/jcm00020-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51b9/265731/043aff6cf1ad/jcm00020-0283-b.jpg

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