Skurrie I J, Head J L, Garland S M
Department of Microbiology, Royal Women's Hospital, Carlton, Victoria, Australia.
J Clin Microbiol. 1991 Aug;29(8):1752-3. doi: 10.1128/jcm.29.8.1752-1753.1991.
An automated microparticle enzyme immunoassay (IMx Rubella IgG Antibody Assay; Abbott Laboratories, North Chicago, Ill.) was compared with a conventional enzyme-linked immunosorbent assay (ELISA) for detection of rubella-specific immunoglobulin G (IgG) in 400 consecutive antenatal patients. There was complete agreement between the two tests in this population, which had a positivity rate of 99% for rubella-specific IgG antibodies. The performance of the IMx was also evaluated at the cutoff zone by assaying 64 selected antenatal serum samples with low or negative rubella antibody titers as determined by ELISA. Overall, the IMx was found to be a specific, sensitive assay for the detection of rubella-specific IgG and is virtually fully automated for easy performance.
采用自动微粒酶免疫分析法（IMx风疹IgG抗体检测法；雅培实验室，伊利诺伊州北芝加哥）与传统酶联免疫吸附测定法（ELISA）对400例连续产前患者检测风疹特异性免疫球蛋白G（IgG）。在该人群中两种检测方法结果完全一致，风疹特异性IgG抗体阳性率为99%。通过检测64份经ELISA测定风疹抗体效价低或为阴性的选定产前血清样本，还对IMx在临界区的性能进行了评估。总体而言，发现IMx是一种检测风疹特异性IgG的特异、灵敏的检测方法，且几乎完全自动化，操作简便。
