基于新型合成肽的DETECT - 风疹酶免疫测定法与Enzygnost和IMx用于检测风疹特异性免疫球蛋白G的比较
Comparison of novel synthetic peptide-based DETECT-RUBELLA enzyme immunoassays with Enzygnost and IMx for detection of rubella-specific immunoglobulin G.
作者信息
Pedneault L, Zrein M, Robillard L, Landry F, Lacroix M, Joncas J
机构信息
Department of Microbiology and Immunology, Université de Montréal, Québec.
出版信息
J Clin Microbiol. 1994 Apr;32(4):1085-7. doi: 10.1128/jcm.32.4.1085-1087.1994.
Abstract
Enzyme immunoassays (EIAs) using synthetic peptides SP-E1 and SP-E1E2 (DETECT-RUBELLA [Bio-Chem]) were compared with two viral lysate-based EIAs (Enzygnost [Behring] and IMx [Abbott]) for the detection of rubella virus-specific immunoglobulin G antibodies. Sensitivities of 94.7, 100, 98.6, and 100% and specificities of 100, 97.4, 100, and 73.7% were found for the SP-E1, SP-E1E2, Enzygnost, and IMx EIAs, respectively.
摘要
使用合成肽SP-E1和SP-E1E2（DETECT-RUBELLA [Bio-Chem]）的酶免疫测定（EIA）与两种基于病毒裂解物的EIA（Enzygnost [贝林]和IMx [雅培]）进行比较，以检测风疹病毒特异性免疫球蛋白G抗体。结果发现，SP-E1、SP-E1E2、Enzygnost和IMx EIA的敏感性分别为94.7%、100%、98.6%和100%，特异性分别为100%、97.4%、100%和73.7%。
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