Fujioka Maiko, Sudo Yumi, Okumura Mai, Wu Jian, Uehara Mariko, Takeda Ken, Hosokawa Yu, Yamada Kazuhiko, Ikegami Sachie, Ishimi Yoshiko
Division of Applied Food Research, National Institute of Health and Nutrition, Tokyo 162-8636, Japan.
Metabolism. 2007 Aug;56(8):1142-8. doi: 10.1016/j.metabol.2007.04.008.
Few studies have examined the effects of isoflavones on bone formation during growth period in male and female animals. In this study, the effects of daidzein or genistein on bone formation were assessed in immature male and female mice. Five-week-old male and female mice were divided respectively into 3 groups (n=8 per group) as follows: control group (C) fed a control diet (AIN-93G), daidzein group (D) fed a control diet containing 0.08% pure daidzein, and genistein group (G) fed a control diet containing 0.08% pure genistein. After 4 weeks, the male D group had a significantly higher bone mineral density (BMD) in whole body, lumbar spine, and femur than did the C group. On the contrary, BMD of the whole body and femur in the female D group was significantly lower than that in the C group. The BMD of the whole femur in the male G group also was significantly higher than for the C group. Histologic analysis revealed that the bone formation rate was significantly higher in the male D and G groups, and lower in the female D group compared with the C group. These results suggest that daidzein has a specific, sexually dimorphic effect on bone formation and BMD during growth period in mice.
很少有研究考察异黄酮对雄性和雌性动物生长期骨形成的影响。在本研究中，评估了大豆苷元和染料木黄酮对未成熟雄性和雌性小鼠骨形成的影响。将5周龄的雄性和雌性小鼠分别分为3组（每组n = 8），如下：对照组（C）喂食对照饮食（AIN - 93G），大豆苷元组（D）喂食含0.08%纯大豆苷元的对照饮食，染料木黄酮组（G）喂食含0.08%纯染料木黄酮的对照饮食。4周后，雄性D组全身、腰椎和股骨的骨矿物质密度（BMD）显著高于C组。相反，雌性D组全身和股骨的BMD显著低于C组。雄性G组整个股骨的BMD也显著高于C组。组织学分析显示，与C组相比，雄性D组和G组的骨形成率显著更高，而雌性D组更低。这些结果表明，大豆苷元对小鼠生长期的骨形成和BMD具有特定的、性别二态性影响。
