Ohtani Junji, Hernandez Rene Arturo Marquez, Sunagawa Hiroko, Fujita Tadashi, Kawata Toshitsugu, Kaku Masato, Motokawa Masahide, Tsuka Natsumi, Koseki Hiroyuki, Matsuda Yayoi, Hayashi Hidetaka, Abedini Sara, Tanne Kazuo
Department of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima-city, Hiroshima, Japan.
Nutr J. 2009 Jul 3;8:30. doi: 10.1186/1475-2891-8-30.
The incidence of primary osteoporosis is higher in Japan than in USA and European countries. Recently, the importance of preventive medicine has been gradually recognized in the field of orthopaedic surgery with a concept that peak bone mass should be increased in childhood as much as possible for the prevention of osteoporosis. Under such background, we have developed a new bean snack with an aim to improve bone volume loss. In this study, we examined the effects of a newly developed snack on bone volume and density in osteoporosis model mice.
Orchiectomy (ORX) and ovariectomy (OVX) were performed for C57BL/6J mice of twelve-week-old (Jackson Laboratory, Bar Harbar, ME, USA) were used in this experiment. We prepared and given three types of powder diet e.g.: normal calcium diet (NCD, Ca: 0.9%, Clea Japan Co., Tokyo, Japan), low calcium diet (LCD, Ca: 0.63%, Clea Japan Co.,) and special diet (SCD, Ca: 0.9%). Eighteen weeks after surgery, all the animals were sacrified and prepared for histomorphometric analysis to quantify bone density and bone mineral content.
As a result of histomorphometric examination, SCD was revealed to enhance bone volume irrespective of age and sex. The bone density was increased significantly in osteoporosis model mice fed the newly developmental snack as compared with the control mice. The bone mineral content was also enhanced significantly. These phenomena were revealed in both sexes.
It is shown that the newly developed bean snack is highly effective for the improvement of bone volume loss irrespective of sex. We demonstrated that newly developmental snack supplements may be a useful preventive measure for Japanese whose bone mineral density values are less than the ideal condition.
日本原发性骨质疏松症的发病率高于美国和欧洲国家。最近，预防医学在骨科领域的重要性逐渐得到认可，其理念是在儿童时期应尽可能增加峰值骨量以预防骨质疏松症。在这样的背景下，我们开发了一种新的豆类零食，旨在改善骨量流失。在本研究中，我们检测了这种新开发的零食对骨质疏松症模型小鼠骨量和骨密度的影响。
对12周龄的C57BL/6J小鼠（美国缅因州巴尔港杰克逊实验室）进行去势手术（ORX）和卵巢切除术（OVX），用于本实验。我们制备并给予三种类型的粉末饲料，即：正常钙饲料（NCD，钙含量：0.9%，日本东京克利尔日本公司）、低钙饲料（LCD，钙含量：0.63%，日本克利尔日本公司）和特殊饲料（SCD，钙含量：0.9%）。手术后18周，所有动物被处死并准备进行组织形态计量分析，以量化骨密度和骨矿物质含量。
组织形态计量学检查结果显示，无论年龄和性别，SCD均可增加骨量。与对照小鼠相比，喂食新开发零食的骨质疏松症模型小鼠的骨密度显著增加。骨矿物质含量也显著提高。这些现象在两性中均有体现。
结果表明，新开发的豆类零食对改善骨量流失具有高效性，且与性别无关。我们证明，新开发的零食补充剂可能是一种对骨矿物质密度值低于理想状态的日本人有用的预防措施。
