Yu Xuping, Zhu Chun, Zheng Xintian, He Shicheng, Liu Xiaoning
Institute of Preventive Veterinary Medicine, College of Animal Sciences, Zhejiang University, 268 Kai Xuan Road, Hangzhou, Zhejiang Province 310029, PR China.
Virus Genes. 2007 Dec;35(3):605-9. doi: 10.1007/s11262-007-0112-1. Epub 2007 Jul 6.
Goose circovirus (GoCV), a potential immuno-suppressive virus, was detected in a sample collected from Yongkang avian influenza (AI) case in Zhejiang Province of eastern China. Further, epidemiological investigation showed that, four farms located in the two major rearing areas of Yongkang and Xiangshan out of eight in Zhejiang Province were positive for GoCV. However, the geese in these GoCV positive farms exhibited no apparent clinical abnormality. The complete genomes of ten isolates from Yongkang AI case (yk1) and other four different farms were sequenced. They had 90.8-99.9% nucleotide (nt) identity with each other and had 91.3-95.8% nt identity to that of the Taiwan and German strains. Genome analysis showed that the GoCVs isolated in Zhejiang Province possessed common features of circovirus, including the potentially replication-associated stem-loop structure, the Rep protein motifs, etc. Highly variable region was also detected between nt27 and nt72 in the intergenic region. Phylogenetic analysis showed that GoCVs could be divided into three distinct genetic groups, where groups I and II could each be further divided into two subgroups (I1, I2 and II1, II2, respectively). No obvious characteristic was found between subclinical strains and clinical strains. However, both the phylogenetic result and analysis of highly variable region revealed that GoCVs isolated from different locations had some geographically dependent characteristics. This is the first epidemiological investigation of GoCV in the mainland of China.
鹅圆环病毒（GoCV）是一种潜在的免疫抑制病毒，在中国东部浙江省永康禽流感病例采集的样本中被检测到。此外，流行病学调查显示，浙江省八个主要养殖地区中的永康和象山两个地区的四个养殖场GoCV呈阳性。然而，这些GoCV阳性养殖场的鹅未表现出明显的临床异常。对来自永康禽流感病例（yk1）和其他四个不同养殖场的十个分离株的全基因组进行了测序。它们彼此之间的核苷酸（nt）同一性为90.8 - 99.9%，与台湾和德国毒株的nt同一性为91.3 - 95.8%。基因组分析表明，在浙江省分离的GoCV具有圆环病毒的共同特征，包括潜在的复制相关茎环结构、Rep蛋白基序等。在基因间隔区的nt27和nt72之间也检测到高变区。系统发育分析表明，GoCV可分为三个不同的遗传组，其中第一组和第二组又可进一步分为两个亚组（分别为I1、I2和II1、II2）。在亚临床毒株和临床毒株之间未发现明显特征。然而，系统发育结果和高变区分析均显示，从不同地点分离的GoCV具有一些地理依赖性特征。这是中国大陆首次对GoCV进行的流行病学调查。
