Becker Stephan, Maissen Otto, Ponomarev Igor, Stoll Thierry, Meury Thomas, Sprecher Christoph, Alini Mauro, Wilke Ingo
Spine Center, Orthopedic Hospital Speising, Speisinger Str. 109, Vienna, Austria.
J Mater Sci Mater Med. 2008 Feb;19(2):659-65. doi: 10.1007/s10856-007-3223-2. Epub 2007 Jul 10.
We investigated the osteopromotive properties of plasmatransglutaminase (F XIII), bone marrow and venous blood on a resorbable beta-tricalcium phosphate (beta-TCP) scaffold. A baseline binding and release study of F XIII from the scaffold showed a continuous release of 18% of the total dose after 48 h. The main study consisted of 18 adult sheep with cylindrical defects in both tibiae. The defects were filled with a beta-TCP cylinder impregnated either with bone marrow, venous blood, F XIII or sheep were treated with 1250 IU F XIII intravenously over 14 days (n = 4 in each group). The defects were left open in two sheep. QCT and histology was performed after 6 and 12 weeks. The best bone ingrowth was seen after 6 weeks in the bone marrow group and after 12 weeks in the local F XIII group. The highest ingrowth on the inside of the cylinder proving the osteopromoting potential of F XIII was found in the local F XIII group. In our opinion F XIII is a good and readily available osteopromoting agent which can be used with beta-TCP in cases of bone deficit to promote bone regeneration.
我们研究了血浆转谷氨酰胺酶（F XIII）、骨髓和静脉血对可吸收β-磷酸三钙（β-TCP）支架的促骨生成特性。对F XIII从支架上的基线结合和释放研究表明，48小时后总剂量的18%持续释放。主要研究包括18只成年绵羊，其双侧胫骨有圆柱形缺损。缺损处填充有浸渍了骨髓、静脉血、F XIII的β-TCP圆柱体，或者绵羊在14天内静脉注射1250 IU F XIII（每组n = 4）。两只绵羊的缺损处保持开放。在6周和12周后进行定量计算机断层扫描（QCT）和组织学检查。骨髓组在6周后观察到最佳的骨长入，局部F XIII组在12周后观察到最佳的骨长入。在局部F XIII组中，在圆柱体内部发现了最高的骨长入，证明了F XIII的促骨生成潜力。我们认为F XIII是一种良好且易于获得的促骨生成剂，在骨缺损情况下可与β-TCP一起使用以促进骨再生。
