Tiwari Vaibhav, Shukla Shripaad Y, Yue Beatrice Y J T, Shukla Deepak
Department of Ophthalmology and Visual Sciences, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
J Gen Virol. 2007 Aug;88(Pt 8):2106-2110. doi: 10.1099/vir.0.82830-0.
Herpes simplex virus type 2 (HSV-2) infections in the eye are becoming increasingly common in adults. The most likely point of entry for HSV-2 into the eye is through the cornea. By using primary cultures of human corneal fibroblasts (CFs), a natural target-cell type for infection, it was demonstrated that CFs are highly susceptible to HSV-2 entry and replication. RT-PCR and flow-cytometry analyses demonstrated expression of herpesvirus entry mediator (HVEM), a known mediator for HSV-2 entry into cells. Blocking of virus entry into CFs by anti-HVEM antibody implicated HVEM as a potential receptor for HSV-2 infection. These results indicate that HVEM may play a crucial role in HSV-2-induced corneal infections.
单纯疱疹病毒2型（HSV-2）眼部感染在成人中越来越常见。HSV-2进入眼睛最可能的途径是通过角膜。利用人角膜成纤维细胞（CFs）的原代培养物（一种感染的天然靶细胞类型），证明CFs对HSV-2的进入和复制高度敏感。逆转录聚合酶链反应（RT-PCR）和流式细胞术分析表明疱疹病毒进入介质（HVEM）有表达，HVEM是已知的HSV-2进入细胞的介质。抗HVEM抗体阻断病毒进入CFs表明HVEM是HSV-2感染的潜在受体。这些结果表明，HVEM可能在HSV-2诱导的角膜感染中起关键作用。
