Wang Zhimin, Yuan Chao, Zhang Shaowei, Tian Shibing, Tang Qinglin, Wei Dayong, Niu Yi
College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.
Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, Chongqing, China.
Front Plant Sci. 2021 Jul 22;12:648193. doi: 10.3389/fpls.2021.648193. eCollection 2021.
Anther indehiscence is an important form of functional male sterility that can facilitate the production of hybrid seeds. However, the molecular mechanisms of anther indehiscence-based male sterility in eggplant ( L.) have not been thoroughly explored. We performed transcriptome sequencing and real-time quantitative reverse transcription-PCR (qRT-PCR) assays to compare the fertile line (F142) and male sterile line (S12) eggplant. We identified 2,670 differentially expressed genes (DEGs) between lines. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses identified 31 DEGs related to hormone biosynthesis. We, therefore, measured phytohormone contents, such as jasmonic acid (JA), auxin (IAA), gibberellin (GA), and abscisic acid (ABA) in S12 and F142. There were differences in IAA, GA, and ABA levels between S12 and F142, while JA levels were significantly lower in S12 than in F142. Five key genes in the JA signaling pathway were differentially expressed in S12 vs. F142. Of these, and were significantly upregulated and , and were downregulated in S12 vs. F142. Protein-protein interaction studies identified a direct interaction between SmDAD1 and SmLOX, while SmDAD1 failed to interact with SmJAR1, SmCOI1, and SmJAZ1. The data represent a valuable resource for further exploration of regulatory mechanisms underlying anther dehiscence in eggplant.
花药不开裂是功能性雄性不育的一种重要形式，有助于杂交种子的生产。然而，茄子花药不开裂型雄性不育的分子机制尚未得到充分探索。我们进行了转录组测序和实时定量逆转录PCR（qRT-PCR）分析，以比较茄子的可育系（F142）和雄性不育系（S12）。我们鉴定出两系之间有2670个差异表达基因（DEG）。基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路分析确定了31个与激素生物合成相关的DEG。因此，我们测定了S12和F142中茉莉酸（JA）、生长素（IAA）、赤霉素（GA）和脱落酸（ABA）等植物激素的含量。S12和F142之间IAA、GA和ABA水平存在差异，而S12中的JA水平显著低于F142。JA信号通路中的五个关键基因在S12与F142中差异表达。其中，在S12与F142中 和 显著上调，而 、 和 下调。蛋白质-蛋白质相互作用研究确定了SmDAD1和SmLOX之间的直接相互作用，而SmDAD1未能与SmJAR1、SmCOI1和SmJAZ1相互作用。这些数据为进一步探索茄子花药开裂的调控机制提供了宝贵资源。
