Gillingwater K, Büscher P, Brun R
Parasite Chemotherapy, Department of Medical Parasitology and Infection Biology, Swiss Tropical Institute, Socinstrasse 57, 4002 Basel, Switzerland.
Vet Parasitol. 2007 Sep 1;148(2):114-21. doi: 10.1016/j.vetpar.2007.05.020. Epub 2007 Jul 10.
The animal pathogenic protozoan, Trypanosoma evansi, leads to a wasting disease in equines, cattle and camels, commonly known as Surra. It is extensively distributed geographically with a wide range of mammalian hosts and causes great economical loss. Trypanosoma equiperdum causes a venereal disease called Dourine in horses and donkeys. Chemotherapy appears to be the most effective form of control for T. evansi, whereas infections caused by T. equiperdum are considered incurable. Due to emerging drug resistance, efficient control of T. evansi is severely threatened, emphasising the urgent need to find new alternative drugs. A drug profile for a panel of T. evansi and T. equiperdum strains has been established for the four standard drugs currently used in treatment. The (3)H-hypoxanthine incorporation assay was used to obtain 50% inhibitory concentration (IC(50)) values for each standard drug against the various strains. The results indicate the presence (and in some cases, the emergence) of drug resistance in several strains. This panel of characterised strains with known drug sensitivities and resistances will be of great value for the screening of new active compounds, in comparison with the four standard drugs currently available.
动物致病性原生动物伊氏锥虫（Trypanosoma evansi）可导致马、牛和骆驼患上一种消瘦病，通常称为苏拉病。它在地理上广泛分布，宿主范围广泛，会造成巨大的经济损失。马媾疫锥虫（Trypanosoma equiperdum）会在马和驴中引发一种称为马媾疫的性病。化疗似乎是控制伊氏锥虫最有效的方法，而由马媾疫锥虫引起的感染则被认为无法治愈。由于新出现的耐药性，对伊氏锥虫的有效控制受到严重威胁，这凸显了寻找新替代药物的迫切需求。已针对目前治疗中使用的四种标准药物建立了一组伊氏锥虫和马媾疫锥虫菌株的药物谱。采用（3）H-次黄嘌呤掺入试验来获取每种标准药物针对各种菌株的50%抑制浓度（IC50）值。结果表明几种菌株中存在（在某些情况下还出现了）耐药性。与目前可用的四种标准药物相比，这组具有已知药物敏感性和耐药性的特征菌株对于筛选新的活性化合物将具有重要价值。
