Koshy Lata, Paris Emma, Ling Sarah, Jones Tim, Bérubé Kelly
School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3US, Wales, UK.
Sci Total Environ. 2007 Oct 1;384(1-3):171-81. doi: 10.1016/j.scitotenv.2007.06.017. Epub 2007 Jul 12.
With the UK producing 400 million tonnes of waste each year, the problem of waste disposal is recognised as one of the most serious environmental problems facing the nation. Of this, over 35 million tonnes is municipal waste, largely derived from households, but also includes some commercial and industrial waste. There are strong national and international concerns about the possible adverse health effects of living in the vicinity of municipal waste landfills. An understanding of the ranges of toxicity of landfill emissions is crucial to determine the degree of concern we should have about the potential effects they could have upon nearby populations and the surrounding environment. Leachates from three different types of landfills have been collected and screened for their potential to induce toxicity. Bioreactivity was measured by a plasmid DNA scission assay (PSA), and 2',7'-dichlorodihydrofluorescin fluorescence (DCFH). The results indicate that leachates cause damage to plasmid DNA in a dose-dependent manner and that toxicity varies between different types of landfills as well as within individual waste sites. Overall, the data implies that the complex chemistry involved in leachate formation has yet to be delineated in terms of the toxicological response.
英国每年产生4亿吨垃圾，垃圾处理问题被认为是该国面临的最严重的环境问题之一。其中，超过3500万吨是城市垃圾，主要来自家庭，但也包括一些商业和工业垃圾。对于生活在城市垃圾填埋场附近可能产生的健康不利影响，国内和国际都极为关注。了解垃圾填埋场排放物的毒性范围对于确定我们应对其可能对附近居民和周围环境产生的潜在影响的关注程度至关重要。已收集了来自三种不同类型垃圾填埋场的渗滤液，并对其诱导毒性的潜力进行了筛选。通过质粒DNA断裂试验（PSA）和2',7'-二氯二氢荧光素荧光（DCFH）测量生物反应性。结果表明，渗滤液以剂量依赖的方式对质粒DNA造成损伤，并且不同类型的垃圾填埋场以及单个垃圾处理场内部的毒性各不相同。总体而言，数据表明渗滤液形成过程中涉及的复杂化学性质在毒理学反应方面尚未得到明确界定。
