在加州罂粟悬浮培养物中，联合诱导子提高苯并菲啶生物碱产量和蛋白质表达。

Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures.

作者信息

Cho Hwa-Young, Lee-Parsons Carolyn W T, Yoon Sung-Yong H, Rhee Hong Soon, Park Jong Moon

机构信息

Advanced Environmental Biotechnology Research Center, School of Environmental Science and Engineering, Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Pohang, 790-784, South Korea.

出版信息

Biotechnol Lett. 2007 Dec;29(12):2001-5. doi: 10.1007/s10529-007-9469-4. Epub 2007 Jul 13.

DOI:10.1007/s10529-007-9469-4

PMID:17628748

Abstract

Production of the benzophenanthridine alkaloids in Eschscholtzia californica suspension cell cultures was optimized by adding 0.5 mg methyl jasmonate (MJ) and 0.02 mg salicylic acid (SA)/g FCW after 7 days cultivation. Sanguinarine reached 24 mg/g DCW by such treatment; 10 times higher than in control cell cultures. MJ and SA induced expression of berberine bridge enzyme and 3'-hydroxy-(S)-N-methylcoclaurine-4'-O-methyltransferase, respectively. MJ plus SA induced over-expression of both enzymes.

摘要

在花菱草悬浮细胞培养物中，通过在培养7天后添加0.5毫克茉莉酸甲酯（MJ）和0.02毫克水杨酸（SA）/克鲜重细胞，优化了苯并菲啶生物碱的生产。通过这种处理，血根碱达到了24毫克/克干重，比对照细胞培养物中的含量高10倍。MJ和SA分别诱导了小檗碱桥酶和3'-羟基-（S）-N-甲基去甲乌药碱-4'-O-甲基转移酶的表达。MJ加SA诱导了这两种酶的过表达。