Kollárová R, Oblozinský M, Kováciková V, Holková I, Balazová A, Pekárová M, Hoffman P, Bezáková L
Pharmazie. 2014 Aug;69(8):637-40.
In this study we investigated the influence of biotic elicitor (phytopathogenic fungus Botrytis cinerea) and abiotic elicitors (methyljasmonate [MJ] and salicylic acid [SA]) on lipoxygenase (LOX) activity and sanguinarine production in cell suspension cultures of California poppy (Eschscholtzia californica CHAM.). We have observed different time effects of elicitors (10, 24, 48 and 72 h) on LOX activity and production of sanguinarine in in vitro cultures. All elicitors used in the experiments evidently increased the LOX activity and sanguinarine production in contrast to control samples. The highest LOX activities were determined in samples elicitated by MJ after 48 h and 72 h and the lowest LOX activities (in contrast to control samples) were detected after biotic elicitation by Botrytis cinerea. These activities showed about 50% lower level against the activities after MJ elicitation. The maximal amount of sanguinarine was observed after 48 h in MJ treated cultures (429.91 mg/g DCW) in comparision with control samples. Although all elicitors affect the sanguinarine production, effect of SA and biotic elicitor on sanguinarine accumulation in in vitrocultures was not so significant than after MJ elicitation.
在本研究中，我们调查了生物诱导剂（植物致病真菌灰葡萄孢）和非生物诱导剂（茉莉酸甲酯[MJ]和水杨酸[SA]）对加利福尼亚罂粟（Eschscholtzia californica CHAM.）细胞悬浮培养物中脂氧合酶（LOX）活性和血根碱产量的影响。我们观察到诱导剂（10、24、48和72小时）对体外培养物中LOX活性和血根碱产量的不同时间效应。与对照样品相比，实验中使用的所有诱导剂均明显提高了LOX活性和血根碱产量。在48小时和72小时后，经MJ诱导的样品中测定出最高的LOX活性，而经灰葡萄孢生物诱导后检测到最低的LOX活性（与对照样品相比）。这些活性比MJ诱导后的活性低约50%。与对照样品相比，在MJ处理的培养物中，48小时后观察到血根碱的最大含量（429.91 mg/g DCW）。尽管所有诱导剂都会影响血根碱的产量，但SA和生物诱导剂对体外培养物中血根碱积累的影响不如MJ诱导后显著。
