Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, Kalinčiakova 8, Bratislava 83232, Slovakia.
Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Odbojárov 10, Bratislava 83232, Slovakia.
Molecules. 2020 Mar 11;25(6):1261. doi: 10.3390/molecules25061261.
Macarpine is a minor benzophenanthridine alkaloid with interesting biological activities, which is produced in only a few species of the Papaveraceae family, including . Our present study was focused on the enhancement of macarpine production in suspension cultures using three elicitation models: salicylic acid (SA) (4; 6; 8 mg/L) elicitation, and simultaneous or sequential combinations of SA and L-tyrosine (1 mmol/L). Sanguinarine production was assessed along with macarpine formation in elicited suspension cultures. Alkaloid production was evaluated after 24, 48 and 72 h of elicitation. Among the tested elicitation models, the SA (4 mg/L), supported by L-tyrosine, stimulated sanguinarine and macarpine production the most efficiently. While sequential treatment led to a peak accumulation of sanguinarine at 24 h and macarpine at 48 h, simultaneous treatment resulted in maximum sanguinarine accumulation at 48 h and macarpine at 72 h. The effect of SA elicitation and precursor supplementation was evaluated also based on the gene expression of 4'-OMT, CYP719A2, and CYP719A3. The gene expression of investigated enzymes was increased at all used elicitation models and their changes correlated with sanguinarine but not macarpine accumulation.
麦克林是一种具有有趣生物活性的次要苯并菲啶生物碱，仅在罂粟科的少数几种物种中产生，包括 。我们目前的研究集中在使用三种诱导模型来提高 悬浮培养物中的麦克林产量：水杨酸（SA）（4；6；8 mg/L）诱导，以及 SA 和 L-酪氨酸的同时或顺序组合（1 mmol/L）。在诱导的悬浮培养物中评估了血根碱和麦克林的形成。在诱导 24、48 和 72 小时后评估生物碱的产生。在测试的诱导模型中，SA（4 mg/L）与 L-酪氨酸一起支持最有效地刺激血根碱和麦克林的产生。虽然顺序处理导致血根碱在 24 小时达到峰值积累，而麦克林在 48 小时达到峰值积累，但同时处理导致血根碱在 48 小时达到最大积累，而麦克林在 72 小时达到最大积累。还根据 4'-OMT、CYP719A2 和 CYP719A3 的基因表达评估了 SA 诱导和前体补充的效果。在所使用的所有诱导模型中，研究酶的基因表达均增加，其变化与血根碱但与麦克林积累相关。
