Wang Aijun, Ao Qiang, Wei Yujun, Gong Kai, Liu Xuesong, Zhao Nanming, Gong Yandao, Zhang Xiufang
Department of Biological Sciences and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, 100084, China.
Biotechnol Lett. 2007 Nov;29(11):1697-702. doi: 10.1007/s10529-007-9460-0. Epub 2007 Jul 13.
Porous fiber-reinforced chitosan nerve conduits were fabricated from chitosan yarns and a chitosan solution by combining an industrial braiding method with a mold casting/lyophilization technique. The conduits were permeable to molecules ranging in molecular size from 180 Da (glucose) to 66,200 Da (BSA). The compressive load of the reinforced conduits was significantly higher than that of a non-reinforced control conduit at equal levels of strain. The tensile strength of the reinforced conduits was also increased from 0.41 +/- 0.17 to 3.69 +/- 0.64 MPa. An in vitro cytotoxicity test showed the conduits were not cytotoxic to Neuro-2a cells. Preliminary in vivo implantation testing indicated that the conduits were compatible with the surrounding tissue.
多孔纤维增强壳聚糖神经导管是通过将工业编织方法与铸模/冻干技术相结合，由壳聚糖纱线和壳聚糖溶液制成的。这些导管对于分子大小从180道尔顿（葡萄糖）到66,200道尔顿（牛血清白蛋白）的分子具有渗透性。在相同应变水平下，增强导管的压缩载荷显著高于未增强的对照导管。增强导管的拉伸强度也从0.41±0.17兆帕增加到3.69±0.64兆帕。体外细胞毒性试验表明，这些导管对Neuro-2a细胞无细胞毒性。初步的体内植入试验表明，这些导管与周围组织相容。
