You Xue-Jun, Lu Xiang, Liu Yang, Liu Zheng, Gao Qi-Xue, Cui Yonghua
Department of Otorhinolaryngology Head and Neck Surgery, Tongfi Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2007 May;42(5):345-9.
To compare the expression difference of Toll-like receptor-2 (TLR-2) and Toll-like receptor-4 (TLR-4) protein and mRNA among the chronic rhinosinusitis tissues, nasal polyps tissues, and normal mucosa tissues.
The mRNA expression of TLR-2 and TLR-4 in chronic rhinosinusitis tissues from 10 patients, in nasal polyp tissues from 10 patients, and in inferior turbinate tissues from 10 patients underwent nasal septum operation was detected by means of reverse transcriptase-polymerase chain reaction (RT-PCR). Immunohistochemistry (IHC) was used to detected the expression of TLR-2 and TLR-4 protein in a different set of 20 chronic rhinosinusitis tissues, 20 nasal polyp tissues, and 20 normal inferior turbinate tissues.
(1) The mRNA and protein expression of TLR-2 and TLR-4 was detected on epithelial and glandular cells membrane in all chronic rhinosinusitis, nasal polyps and control tissues . (2) The mRNA and protein expression of TLR-2 and the mRNA expression of TLR-4 in chronic rhinosinusitis tissues was significantly increased compared with that in nasal polyps and control tissues (P < 0.05). (3) The expression intensity of TLR-2 and TLR-4 mRNA and protein between nasal polyps and control tissues was found no significant difference (P > 0.05).
Different TLR-2 and TLR-4 protein and mRNA level in chronic rhinosinusitis and nasal polyp tissues might imply that TLR-2 and TLR-4 play different role in the pathogenesis of chronic rhinosinusitis and nasal polyps.
比较慢性鼻窦炎组织、鼻息肉组织和正常黏膜组织中Toll样受体2（TLR-2）和Toll样受体4（TLR-4）蛋白及mRNA的表达差异。
采用逆转录聚合酶链反应（RT-PCR）检测10例慢性鼻窦炎患者的鼻窦组织、10例鼻息肉患者的鼻息肉组织以及10例行鼻中隔手术患者的下鼻甲组织中TLR-2和TLR-4的mRNA表达。采用免疫组织化学法（IHC）检测另一组20例慢性鼻窦炎组织、20例鼻息肉组织和20例正常下鼻甲组织中TLR-2和TLR-4蛋白的表达。
（1）在所有慢性鼻窦炎、鼻息肉及对照组织的上皮细胞和腺细胞膜上均检测到TLR-2和TLR-4的mRNA及蛋白表达。（2）慢性鼻窦炎组织中TLR-2的mRNA及蛋白表达和TLR-4的mRNA表达较鼻息肉及对照组织显著升高（P<0.05）。（3）鼻息肉组织与对照组织中TLR-2和TLR-4的mRNA及蛋白表达强度差异无统计学意义（P>0.05）。
慢性鼻窦炎和鼻息肉组织中TLR-2和TLR-4蛋白及mRNA水平不同，这可能意味着TLR-2和TLR-4在慢性鼻窦炎和鼻息肉的发病机制中发挥不同作用。
