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高等植物中单倍体的复兴。

The resurgence of haploids in higher plants.

作者信息

Forster Brian P, Heberle-Bors Erwin, Kasha Ken J, Touraev Alisher

机构信息

SCRI, Genetics Programme, Invergowrie, Dundee, DD2 5DA, UK.

出版信息

Trends Plant Sci. 2007 Aug;12(8):368-75. doi: 10.1016/j.tplants.2007.06.007. Epub 2007 Jul 12.

Abstract

The life cycle of plants proceeds via alternating generations of sporophytes and gametophytes. The dominant and most obvious life form of higher plants is the free-living sporophyte. The sporophyte is the product of fertilization of male and female gametes and contains a set of chromosomes from each parent; its genomic constitution is 2n. Chromosome reduction at meiosis means cells of the gametophytes carry half the sporophytic complement of chromosomes (n). Plant haploid research began with the discovery that sporophytes can be produced in higher plants carrying the gametic chromosome number (n instead of 2n) and that their chromosome number can subsequently be doubled up by colchicine treatment. Recent technological innovations, greater understanding of underlying control mechanisms and an expansion of end-user applications has brought about a resurgence of interest in haploids in higher plants.

摘要

植物的生命周期通过孢子体和配子体世代交替进行。高等植物中占主导地位且最明显的生命形式是自由生活的孢子体。孢子体是雄雌配子受精的产物,包含来自每个亲本的一组染色体;其基因组构成是2n。减数分裂时染色体数目减半意味着配子体的细胞携带的染色体数是孢子体染色体数的一半(n)。植物单倍体研究始于发现高等植物中携带配子染色体数(n而非2n)的植株能够产生孢子体,并且其染色体数随后可以通过秋水仙素处理加倍。最近的技术创新、对潜在控制机制的更深入理解以及终端用户应用的扩展,使得人们对高等植物单倍体的兴趣再度兴起。

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