Liu Yiqian, Elshan Musazade, Li Geng, Han Xiao, Chen Xiao, Feng Xianzhong
Agronomy College, Jilin Agricultural University, Changchun 130118, China.
Key Laboratory of Soybean Molecular Design Breeding, National Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China.
Int J Mol Sci. 2025 Jan 29;26(3):1154. doi: 10.3390/ijms26031154.
Genome editing-mediated haploid inducer systems (HISs) present a promising strategy for enhancing breeding efficiency in legume crops, which are vital for sustainable agriculture due to their nutritional benefits and ability to fix nitrogen. Traditional legume breeding is often slow and complicated by the complexity of legumes' genomes and the challenges associated with tissue culture. Recent advancements have broadened the applicability of HISs in legume crops, facilitating a reduction in the duration of the breeding cycle. By integrating genome editing technology with haploid breeding systems, researchers can achieve precise genetic modifications and rapidly produce homozygous lines, thereby significantly accelerating the development of desired traits. This review explores the current status and future prospects of genome editing-mediated HISs in legumes, emphasizing the mechanisms of haploid induction; recent breakthroughs; and existing technical challenges. Furthermore, we highlight the necessity for additional research to optimize these systems across various legume species, which has the potential to greatly enhance breeding efficiency and contribute to the sustainability of legume production.
基因组编辑介导的单倍体诱导系统（HISs）为提高豆类作物的育种效率提供了一种很有前景的策略，由于其营养价值和固氮能力，豆类作物对可持续农业至关重要。传统的豆类育种往往进展缓慢，且因豆类基因组的复杂性以及与组织培养相关的挑战而变得复杂。最近的进展拓宽了HISs在豆类作物中的适用性，有助于缩短育种周期。通过将基因组编辑技术与单倍体育种系统相结合，研究人员可以实现精确的基因修饰并快速产生纯合系，从而显著加速所需性状的开发。本综述探讨了基因组编辑介导的HISs在豆类中的现状和未来前景，重点介绍了单倍体诱导的机制、最近的突破以及现有的技术挑战。此外，我们强调有必要进行更多研究，以优化这些系统在各种豆类物种中的应用，这有可能大大提高育种效率，并有助于豆类生产的可持续性。
