Reverberi Roberto, Govoni Maurizio, Verenini Marina
Servizio Trasfusionale, Arcispedale S. Anna, Corso Giovecca 203, 44100 Ferrara, Italy.
Ann Ist Super Sanita. 2007;43(2):176-85.
The irradiation of blood components with X or gamma rays is necessary to prevent the graft-versus-host disease, but it also provokes untoward effects. In particular, red cells are damaged and have a decreased in vivo recovery, an increased in vitro haemolysis, and a leakage of potassium in the supernatant. The results of the clinical studies show that the loss of viability progressively increases with the storage after irradiation. On the other hand, the storage before irradiation is inconsequential. The mechanism through which irradiation causes the loss of viability is unknown, but a critical examination of the literature and our results indicate that the erythrocyte deformability is the only parameter related to viability to show sufficiently precocious and important changes. We also tried to identify the mechanism by which irradiation influences deformability and examined, in particular, the changes in the mean cell volume (MCV) and vesiculation. However, the temporal behaviour of both suggests no causal relationship.
用X射线或γ射线照射血液成分对于预防移植物抗宿主病是必要的，但这也会引发不良影响。特别是，红细胞会受到损伤，体内回收率降低，体外溶血增加，上清液中钾泄漏。临床研究结果表明，照射后随着储存时间的延长，活力丧失逐渐增加。另一方面，照射前的储存则无关紧要。照射导致活力丧失的机制尚不清楚，但对文献和我们的结果进行严格审查表明，红细胞变形性是与活力相关的唯一参数，显示出足够早熟和重要的变化。我们还试图确定照射影响变形性的机制，特别是检查平均细胞体积（MCV）和囊泡形成的变化。然而，两者的时间行为均未表明存在因果关系。
