Luo Jie, Sun Yuyu
Department of Human Ecology, The University of Texas at Austin, Austin, Texas 78712, USA.
J Biomed Mater Res A. 2008 Mar 1;84(3):631-42. doi: 10.1002/jbm.a.31301.
In this study, the surfaces of polypropylene tubing were hydroxylated with potassium persulfate. The resultant tubing surfaces were grafted with methacrylamide (MAA) using ceric(IV) ammonium nitrate as an initiator. Upon chlorination treatment with diluted chlorine bleach, some of the amide groups in the grafted MAA side chains were transformed into stable acyclic N-halamines. The reactions were confirmed with attenuated total reflectance infrared, X-ray photoelectron spectra, and iodimetric titration. The resultant tubing was challenged with Pseudomonas aeruginosa (P. aeruginosa) in a continuous flowing model. Bacteria culturing and scanning electron microscope studies showed that the chlorinated MAA-grafted tubing could provide potent and rechargeable biofilm-controlling functions against the test microorganisms.
在本研究中，聚丙烯管材表面用过硫酸钾进行羟基化处理。所得管材表面以硝酸铈铵为引发剂，接枝甲基丙烯酰胺（MAA）。在用稀释的含氯漂白剂进行氯化处理后，接枝的MAA侧链中的一些酰胺基团转化为稳定的无环N-卤胺。通过衰减全反射红外光谱、X射线光电子能谱和碘量滴定法对反应进行了确认。所得管材在连续流动模型中用铜绿假单胞菌（P. aeruginosa）进行挑战试验。细菌培养和扫描电子显微镜研究表明，氯化接枝MAA的管材对受试微生物可提供有效的、可再充电的生物膜控制功能。
