Jiang Fuguang, Deng Ying, Yeh Chih-Ko, Sun Yuyu
Department of Chemistry, University of Massachusetts, Lowell, MA 01854, USA, ; Tel: +1 978 934 3637,
Biomedical Engineering Program, University of South Dakota, Sioux Falls, SD 57107, USA, ; Tel:+1 605 367 7775,
J Mater Chem B. 2014 Dec 28;2(48):8518-8527. doi: 10.1039/C4TB01131G.
Quaternized chitosans, N,N,N-trimethylchitosans (TMC) with different degree of quaternization were synthesized by reacting methyl iodide with chitosan. The reaction was confirmed by FT-IR and H-NMR characterization. Antimicrobial assay showed that the prepared TMC had potent biocidal effects against planktonic Gram-positive bacteria , Gram-negative bacteria , and yeast . Bacterial and fungal biofilms were formed on poly(methyl methacrylate) (PMMA) films and then treated with TMC aqueous solutions. Zeta potential measurement suggested that TMC bonded onto the preexisting biofilms. Biofilm-binding kinetics was evaluated in UV studies using phenyl group-labeled TMC as model compounds, which revealed that quaternized chitosans bonded onto the preexisting biofilms rapidly. Colony-forming unit (CFU) determination and SEM, confocal laser scanning microscopy (CLSM) and fluorescence microscopy studies demonstrated that the bonded TMC had powerful biocidal activities to eradicate the pre-attached bacterial and fungal cells in the preexisting biofilms. The biocompatibility of the TMC samples with rat skin fibroblast cells was evaluated in the MTT assay.
通过使壳聚糖与碘甲烷反应合成了具有不同季铵化程度的季铵化壳聚糖，即N，N，N-三甲基壳聚糖（TMC）。通过傅里叶变换红外光谱（FT-IR）和氢核磁共振（H-NMR）表征证实了该反应。抗菌试验表明，制备的TMC对浮游的革兰氏阳性菌、革兰氏阴性菌和酵母菌具有强大的杀菌作用。在聚甲基丙烯酸甲酯（PMMA）膜上形成细菌和真菌生物膜，然后用TMC水溶液处理。ζ电位测量表明TMC结合到预先存在的生物膜上。使用苯基标记的TMC作为模型化合物，通过紫外研究评估生物膜结合动力学，结果表明季铵化壳聚糖能迅速结合到预先存在的生物膜上。菌落形成单位（CFU）测定以及扫描电子显微镜（SEM）、共聚焦激光扫描显微镜（CLSM）和荧光显微镜研究表明，结合的TMC具有强大的杀菌活性，可根除预先存在的生物膜中预先附着的细菌和真菌细胞。通过MTT试验评估了TMC样品与大鼠皮肤成纤维细胞的生物相容性。
