Lewenhofer Verena, Schweighofer Lisa, Ledermüller Tobias, Eichsteininger Julia, Kählig Hanspeter, Zehl Martin, Nguyen Chi H, Krupitza Georg, Özmen Ali, Krenn Liselotte
Department of Pharmacognosy, Faculty of Life Sciences, University of Vienna, Vienna, Austria.
Clinical Institute of Pathology, Medical University of Vienna, Vienna, Austria.
Front Pharmacol. 2018 Apr 3;9:304. doi: 10.3389/fphar.2018.00304. eCollection 2018.
A detannified methanolic extract of L. attenuated the formation of cancer cell-induced circular chemorepellent induced defects (CCIDs) in the lymph endothelial cell barrier, which resemble entry ports for the intravasating tumor into the vasculature as a prerequisite for lymph node metastasis. Therefore, the composition of this extract was studied in an activity-guided approach. Since no data on the secondary metabolites of this plant were available, first phytochemical data were collected in the course of the fractionation of the extract. The study resulted in the identification of 14 substances, among them very rare iridoids, such as scrovalentinoside or koelzioside, and several flavonoids (e.g., nepitrin and homoplantaginin). One of the latter group, 2″--acetyl-homoplantaginin, is a new natural compound. In the most active fraction, the flavonoid hispidulin was identified as major component and the assay of the pure compound confirmed a contribution of hispidulin to the CCID-inhibitory effects of . The activity of the two major iridoids in this assay was less compared to hispidulin.
衰弱龙葵的脱鞣甲醇提取物可抑制癌细胞诱导的淋巴内皮细胞屏障中的圆形化学排斥诱导缺陷（CCIDs）的形成，这些缺陷类似于肿瘤浸润进入脉管系统的入口，是淋巴结转移的先决条件。因此，采用活性导向法对该提取物的成分进行了研究。由于尚无关于该植物次生代谢产物的数据，因此在提取物分级分离过程中首先收集了植物化学数据。该研究鉴定出了14种物质，其中包括非常罕见的环烯醚萜类化合物，如scrovalentinoside或koelzioside，以及几种黄酮类化合物（如nepitrin和高车前苷）。后一组中的一种，2″-乙酰基-高车前苷是一种新的天然化合物。在活性最高的馏分中，黄酮类化合物水犀草素被鉴定为主要成分，对纯化合物的测定证实了水犀草素对衰弱龙葵CCID抑制作用的贡献。在该试验中，两种主要环烯醚萜类化合物的活性低于水犀草素。
