Finocchiaro L M, Nahmod V E, Launay J M
Laboratorio de Sustancias Vasoactives, Universidad de Buenos Aires, Argentina.
Biochem J. 1991 Dec 15;280 ( Pt 3)(Pt 3):727-31. doi: 10.1042/bj2800727.
Cultured human peripheral blood mononuclear leucocytes (PBML) were able to synthesize indoleamines, including melatonin, and were also able to convert melatonin taken up from the incubation medium into N-acetyl-5-hydroxytryptamine (NAHT) and 5-hydroxytryptamine (5-HT). These compounds were analysed by h.p.l.c., and melatonin was additionally characterized by two-dimensional t.l.c., mass spectrometry and radioimmunoassay. Only hydroxyindoles were detected by h.p.l.c. in unstimulated PBML culture. Sustained stimulation by melatonin or interferon-gamma (IFN-gamma) increased markedly the basal production of 5-HT. IFN-gamma- or 5-HT-stimulated (but not resting) cells produced NAHT and melatonin. Furthermore, the addition of melatonin to the culture medium strongly enhanced NAHT and 5-HT production without affecting tryptophan hydroxylation, suggesting the possibility of direct or indirect transformation of melatonin into NAHT and 5-HT.
培养的人外周血单个核白细胞（PBML）能够合成包括褪黑素在内的吲哚胺，并且还能够将从培养液中摄取的褪黑素转化为N - 乙酰 - 5 - 羟色胺（NAHT）和5 - 羟色胺（5 - HT）。这些化合物通过高效液相色谱法（h.p.l.c.）进行分析，褪黑素还通过二维薄层层析法（t.l.c.）、质谱分析法和放射免疫分析法进行表征。在未受刺激的PBML培养物中，通过高效液相色谱法仅检测到羟基吲哚。褪黑素或干扰素 - γ（IFN - γ）的持续刺激显著增加了5 - HT的基础产量。IFN - γ或5 - HT刺激的（而非静止的）细胞产生NAHT和褪黑素。此外，向培养基中添加褪黑素强烈增强了NAHT和5 - HT的产生，而不影响色氨酸羟化，这表明褪黑素可能直接或间接转化为NAHT和5 - HT。
