Finocchiaro L M, Polack E, Nahmod V E, Glikin G C
Laboratorio de Sustancias Vasoactivas, Universidad de Buenos Aires, Argentina.
Life Sci. 1995;57(6):559-69. doi: 10.1016/0024-3205(95)00306-q.
Cultured human peripheral blood mononuclear leukocytes [PBML] from patients with anorexia nervosa [AN] did not respond to light stimulation as PBML of normal controls [NC] did. During winter, visible light increased [3H]thymidine incorporation into DNA of NC-PBML stimulated with phytohemagglutinin [PHA]. This effect was enhanced by 10(-7) M melatonin. PHA-stimulated DNA synthesis of PBML from AN patients failed to respond to photic stimulation during winter, and their proliferative response to melatonin was significantly blunted. In vitro photic stimulation of NC-PBML reduced melatonin while increasing both serotonin and 5-hydroxyindole 3-acetic acid [HIAA] production in both basal and PHA-stimulated conditions. In contrast AN-PBML, that in darkness enhanced the oxidative deamination of serotonin into HIAA more than NC-PBML, did not switch their indole metabolism in response to light. Light did not inhibit the binding of both melatonin and serotonin to AN-PBML as occurred in NC-PBML. The present data suggest that AN-PBML do not respond to light in vitro, because of a failure in the regulation of serotonin and melatonin metabolism.
神经性厌食症（AN）患者的培养人外周血单个核白细胞（PBML）对光刺激没有正常对照（NC）的PBML那样的反应。在冬季，可见光增加了用植物血凝素（PHA）刺激的NC-PBML的[3H]胸腺嘧啶核苷掺入DNA的量。这种作用被10^(-7) M褪黑素增强。冬季期间，AN患者的PBML经PHA刺激的DNA合成对光刺激没有反应，并且它们对褪黑素的增殖反应明显减弱。在基础和PHA刺激条件下，对NC-PBML进行体外光刺激会减少褪黑素，同时增加血清素和5-羟吲哚-3-乙酸（HIAA）的产生。相比之下，在黑暗中比NC-PBML更能增强血清素氧化脱氨生成HIAA的AN-PBML，对光没有改变其吲哚代谢。光不像在NC-PBML中那样抑制褪黑素和血清素与AN-PBML的结合。目前的数据表明，由于血清素和褪黑素代谢调节失败，AN-PBML在体外对光没有反应。
