Ogura K, Nishiyama T, Okada T, Kajital J, Narihata H, Watabe T, Hiratsuka A, Watabe T
Department of Hygienic Chemistry, Tokyo College of Pharmacy, Japan.
Biochem Biophys Res Commun. 1991 Dec 31;181(3):1294-300. doi: 10.1016/0006-291x(91)92079-y.
A cDNA containing the entire coding sequence for the subunit protein of rat liver class theta glutathione S-transferase (GST) Yrs-Yrs was isolated from a rat liver lambda gt11 cDNA library. The cDNA, designated GST theta-1, consisted of 1,258 bp which had an open reading frame of 732 bp encoding a polypeptide of 244 amino acid (AA) residues, including the leading AA Met to be removed on expression. The authenticity of the cDNA structure was supported by matching its deduced AA sequence with N-termini of Yrs and peptides obtained thereof by tryptic digestion as well as by CNBr cleavage. The deduced AA sequence of the subunit Yrs (M.W. 27,311) had only a weak homology (19-23%) with those of rat liver classes alpha, mu, and pi GST isozymes. Thus, the first evidence for the molecular cloning of the class theta GST was provided.
从大鼠肝脏λgt11 cDNA文库中分离出一个包含大鼠肝脏θ类谷胱甘肽S-转移酶（GST）Yrs-Yrs亚基蛋白完整编码序列的cDNA。该cDNA命名为GST theta-1，由1258个碱基对组成，有一个732个碱基对的开放阅读框，编码一个244个氨基酸（AA）残基的多肽，包括表达时将被去除的起始氨基酸Met。通过将其推导的AA序列与Yrs的N端以及通过胰蛋白酶消化和溴化氰裂解获得的肽段进行匹配，支持了cDNA结构的真实性。亚基Yrs（分子量27311）的推导AA序列与大鼠肝脏α、μ和π类GST同工酶的序列只有较弱的同源性（19%-23%）。因此，提供了θ类GST分子克隆的首个证据。
