非同源末端连接（NHEJ）衍生的相互染色体易位的形成不需要Ku70。
Formation of NHEJ-derived reciprocal chromosomal translocations does not require Ku70.
作者信息
Weinstock David M, Brunet Erika, Jasin Maria
机构信息
Department of Medicine, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA.
出版信息
Nat Cell Biol. 2007 Aug;9(8):978-81. doi: 10.1038/ncb1624.
Abstract
Chromosomal translocations in lymphoid tumours can involve antigen-receptor loci undergoing V(D)J recombination. Here, we show that translocations are recovered from the joining of RAG-generated double-strand breaks (DSBs) on one chromosome to an endonuclease-generated DSB on a second chromosome, providing evidence for the participation of non-RAG DSBs in some lymphoid translocations. Surprisingly, translocations are increased in cells deficient for the nonhomologous end-joining (NHEJ) protein Ku70, implicating non-canonical joining pathways in their etiology.
摘要
淋巴肿瘤中的染色体易位可涉及经历V(D)J重组的抗原受体基因座。在此，我们表明，易位是通过将一条染色体上RAG产生的双链断裂（DSB）与第二条染色体上核酸内切酶产生的DSB连接而恢复的，这为非RAG DSB参与某些淋巴易位提供了证据。令人惊讶的是，在非同源末端连接（NHEJ）蛋白Ku70缺陷的细胞中易位增加，这表明非经典连接途径在其病因学中起作用。
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