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巢式聚合酶链反应检测少量蓝氏贾第鞭毛虫包囊的灵敏度

Sensitivity of nested PCR in the detection of low numbers of Giardia lamblia cysts.

作者信息

Miller Kathryn M, Sterling Charles R

机构信息

University of Arizona, Department of Veterinary Science and Microbiology, Tucson, AZ 85721, USA.

出版信息

Appl Environ Microbiol. 2007 Sep;73(18):5949-50. doi: 10.1128/AEM.00668-07. Epub 2007 Jul 20.

DOI:10.1128/AEM.00668-07
PMID:17644646
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2074905/
Abstract

Nested PCR was performed on individually isolated Giardia lamblia cysts in replicates of 50 for sets of 1, 2, 3, 4, 5, 7, and 10 cysts. Amplification ranged from 80% for 1 cyst to 100% for 10 cysts. The results suggest that nested PCR is well adapted for G. lamblia single-cyst detection.

摘要

对单个分离出的蓝氏贾第鞭毛虫囊肿进行巢式聚合酶链反应（Nested PCR），每组分别对1、2、3、4、5、7和10个囊肿进行50次重复操作。扩增率从1个囊肿的80%到10个囊肿的100%不等。结果表明，巢式PCR非常适合蓝氏贾第鞭毛虫单囊肿检测。

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