Zhou Xinghua, Pan Wenwen, Li Na, Salah Mahmoud, Guan Shuoning, Li Xiaolan, Wang Yun
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Department of Environmental Agricultural Science, Faculty of Graduate Studies and Environmental Research, Ain Shams University, Cairo 11566, Egypt.
Foods. 2024 Aug 16;13(16):2550. doi: 10.3390/foods13162550.
Lomefloxacin (LOM), an antibiotic crucial for preventing various animal diseases in animal husbandry, can pose serious health risks when found in excessive amounts in meat products. The development of highly specific and sensitive colloidal gold immunochromatographic test strips is essential for the accurate detection of this class of antibiotics. Our study utilized a monoclonal antibody (mAb) assay and immunochromatographic strips to detect lomefloxacin residues in meat products. The results showed minimal cross-reactivity with other structural analogs, with a maximum half inhibitory concentration (IC) of 0.93 ng/mL and a linear range of 0.38 to 2.3 ng/mL for the indirect competitive enzyme-linked immunosorbent assay (ic-ELISA). The recovery of LOM was 80% to 120%, with an average coefficient of variation below 5%. The immunochromatographic strip test results showed a visual detection limit of 2.5 ng/g, meeting the market requirements for the test. This study highlights the significance of specific and sensitive testing methods for detecting lomefloxacin, ensuring consumers' safety and health.
洛美沙星（LOM）是一种在畜牧业中对预防各种动物疾病至关重要的抗生素，当在肉类产品中过量存在时会对健康构成严重风险。开发高度特异性和灵敏的胶体金免疫层析试纸条对于准确检测这类抗生素至关重要。我们的研究利用单克隆抗体（mAb）检测法和免疫层析试纸条来检测肉类产品中的洛美沙星残留。结果显示与其他结构类似物的交叉反应极小，间接竞争酶联免疫吸附测定（ic-ELISA）的最大半数抑制浓度（IC）为0.93 ng/mL，线性范围为0.38至2.3 ng/mL。洛美沙星的回收率为80%至120%，平均变异系数低于5%。免疫层析试纸条检测结果显示视觉检测限为2.5 ng/g，满足市场检测要求。本研究突出了用于检测洛美沙星的特异性和灵敏检测方法的重要性，确保了消费者的安全与健康。
