Wang Wei, Wang Jingneng, Wang Min, Shen Juan
Key Laboratory of Meat Processing and Quality Control, MOE, Key Laboratory of Meat Processing, MARA, Jiangsu Synergetic Innovation Center of Meat Processing and Quality Control, Nanjing Agricultural University, No. 1 Weigang, Nanjing, Jiangsu 210095, China.
Shanghai Xiongtu Biotechnology Co., Ltd., No. 21 Guangfulin Road, Shanghai 201600, China.
Int J Anal Chem. 2021 Mar 9;2021:6633016. doi: 10.1155/2021/6633016. eCollection 2021.
Immunochromatographic assays are good analytical tools for the detection of drug residues. We report a nanosphere-based time-resolved fluorescence immunoassay (nano-TRFIA) based on a monoclonal antibody and a portable TRFIA analyzer for the rapid quantification of chlorpromazine (CPZ) residues in pork. Under optimal conditions, the nano-TRFIA detected CPZ residues within 6 min of sample pretreatment. The results showed good linearity ( = 0.991), with a limit of detection (LOD) of 0.32 g/kg, a wide dynamic range of 0.46-10.0 g/kg, and coefficients of variation (CVs) of the overall intrabatch and interbatch assays of 7.34% and 7.65%, respectively. The nano-TRFIA was also used to detect CPZ at different spiked concentrations in pork, and the results were confirmed via ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The nano-TRFIA was evaluated for the analysis of six commercial pork samples, and the results agreed well with those obtained via UPLC-MS/MS, without significant differences ( > 0.05). Therefore, the proposed nano-TRFIA is a powerful alternative for the rapid and accurate quantification of CPZ residues in pork to meet the required Chinese maximum residue limits for veterinary drugs in foods.
免疫层析测定法是检测药物残留的良好分析工具。我们报道了一种基于单克隆抗体的纳米球时间分辨荧光免疫测定法（nano-TRFIA）以及一种用于快速定量猪肉中氯丙嗪（CPZ）残留的便携式TRFIA分析仪。在最佳条件下，nano-TRFIA在样品预处理后6分钟内即可检测到CPZ残留。结果显示出良好的线性关系（r = 0.991），检测限（LOD）为0.32 μg/kg，动态范围宽达0.46 - 10.0 μg/kg，批内和批间测定的变异系数（CVs）分别为7.34%和7.65%。nano-TRFIA还用于检测猪肉中不同加标浓度的CPZ，结果通过超高效液相色谱 - 串联质谱法（UPLC-MS/MS）得到证实。对nano-TRFIA用于分析六个市售猪肉样品进行了评估，结果与UPLC-MS/MS获得的结果吻合良好，无显著差异（P > 0.05）。因此，所提出的nano-TRFIA是快速准确地定量猪肉中CPZ残留以满足中国食品中兽药最大残留限量要求的有力替代方法。
